Institute for Advanced Ceramics, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, P. R. China.
Key Laboratory of Bio-based Material Science and Technology, Ministry of Education, Northeast Forestry University, Harbin 150040, P. R. China.
ACS Appl Mater Interfaces. 2024 Jun 5;16(22):28991-29002. doi: 10.1021/acsami.4c01382. Epub 2024 May 20.
Triphenylphosphine functionalized carbon dots (TPP-CDs) showcase robust mitochondria targeting capacity owing to their positive electrical properties. However, TPP-CDs typically involve complicated synthesis steps and time-consuming postmodification procedures. Especially, the one-step target-oriented synthesis of TPP-CDs and the regulation of TPP linkage modes remain challenges. Herein, we propose a free-radical-initiated random copolymerization in combination with hydrothermal carbonation to regulate the TPP backbone linkage for target-oriented synthesis of triphenylphosphine copolymerization carbon dots (TPP-CDs). The linkage mechanism of random copolymerization reactions is directional, straightforward, and controllable. The TPP content and IC of hydroxyl radicals scavenging ability of TPP-CDs are 53 wt % and 0.52 mg/mL, respectively. TPP serves as a charge control agent to elevate the negatively charged CDs by 20 mV. TPP-CDs with negative charge can target mitochondria, and in the corresponding mechanism the TPP moiety plays a crucial role in targeting mitochondria. This discovery provides a new perspective on the controlled synthesis, TPP linkage modes, and mitochondrial targeting design of TPP-CDs.
三苯基膦功能化碳点（TPP-CDs）由于其正电性而具有强大的靶向线粒体能力。然而，TPP-CDs 通常涉及复杂的合成步骤和耗时的后修饰过程。特别是，TPP-CDs 的一步靶向合成和 TPP 连接方式的调节仍然是挑战。在此，我们提出了一种自由基引发的无规共聚反应与水热碳化相结合的方法，用于调节 TPP 主链连接方式，以实现靶向合成三苯基膦共聚碳点（TPP-CDs）。无规共聚反应的连接机制具有方向性、直接性和可控性。TPP-CDs 的 TPP 含量和羟基自由基清除能力的 IC50 分别为 53wt%和 0.52mg/mL。TPP 作为电荷控制剂将带负电荷的 CDs 升高 20mV。带负电荷的 TPP-CDs 可以靶向线粒体，在相应的机制中，TPP 部分在靶向线粒体中起着关键作用。这一发现为 TPP-CDs 的可控合成、TPP 连接方式和线粒体靶向设计提供了新的视角。
