Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Saitama, Japan.
Sci Rep. 2024 May 20;14(1):11442. doi: 10.1038/s41598-024-61726-8.
The global supply of fluoropolymers and fluorinated solvents is decreasing due to environmental concerns regarding polyfluoroalkyl substances. CYTOP has been used for decades primarily as a component of a femtoliter chamber array for digital bioanalysis; however, its supply has recently become scarce, increasing the urgency of fabricating a femtoliter chamber array using alternative materials. In this study, we investigated the feasibility of fabricating a femtoliter chamber array using four types of fluoropolymers in stable supply as candidate substitutes and verified their applicability for digital bioanalysis. Among these candidates, Fluorine Sealant emerged as a viable option for fabricating femtoliter chamber arrays using a conventional photolithography process. To validate its efficacy, we performed various digital bioanalysis using FP-A-based chamber arrays with model enzymes such as CRISPR-Cas, horseradish peroxidase, and β-galactosidase. The results demonstrated the similar performance to that of CYTOP, highlighting the broader utility of FP-A in digital bioanalysis. Our findings underscore the potential of FP-A to enhance the versatility of digital bioanalysis and foster the ongoing advancement of innovative diagnostic technologies.
由于人们对全氟烷基物质的环境问题的关注，全球氟聚合物和氟化溶剂的供应正在减少。CYTOP 已经使用了几十年，主要作为用于数字生物分析的皮升室阵列的一个组成部分；然而，其供应最近变得稀缺，这增加了使用替代材料制造皮升室阵列的紧迫性。在这项研究中，我们研究了使用四种稳定供应的氟聚合物制造皮升室阵列的可行性，将它们作为候选替代品，并验证了它们在数字生物分析中的适用性。在这些候选物中，氟密封剂作为使用传统光刻工艺制造皮升室阵列的可行选择脱颖而出。为了验证其功效，我们使用基于 FP-A 的腔室阵列和模型酶（如 CRISPR-Cas、辣根过氧化物酶和β-半乳糖苷酶）进行了各种数字生物分析。结果表明，其性能与 CYTOP 相似，突出了 FP-A 在数字生物分析中的更广泛应用。我们的研究结果强调了 FP-A 增强数字生物分析多功能性的潜力，并促进了创新诊断技术的不断发展。
