Rissin David M, Walt David R
Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, Massachusetts 02155, USA.
J Am Chem Soc. 2006 May 17;128(19):6286-7. doi: 10.1021/ja058425e.
In this communication, single molecules of beta-galactosidase were captured on a 1 mm femtoliter array using biotin-streptavidin binding. The femtoliter arrays, containing 24 000 individual reaction chambers, permit digital concentration readout as the percentage of reaction vessels that successfully capture a target molecule is correlated to the bulk target concentration. This capture and readout approach should prove useful for DNA and antibody assays that utilize an enzyme label to catalyze the generation of a fluorescent signal.
在本通讯中，利用生物素-链霉亲和素结合，将单个β-半乳糖苷酶分子捕获在1毫米飞升阵列上。该飞升阵列包含24000个独立的反应室，由于成功捕获目标分子的反应容器百分比与整体目标浓度相关，因此可以进行数字浓度读数。这种捕获和读数方法对于利用酶标记催化荧光信号产生的DNA和抗体检测应该是有用的。
