Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, Italy.
Institute of Sciences of Food Production, Bari, Italy.
Pest Manag Sci. 2024 Sep;80(9):4585-4593. doi: 10.1002/ps.8164. Epub 2024 May 20.
Xylella fastidiosa is an important causative agent of Olive Quick Decline Syndrome in the Apulia region of Italy. The current study evaluated the bioefficacy of three entomopathogenic fungal strains: Beauveria bassiana SGB7004, Metarhizium robertsii SGB1K, and Akanthomyces lecanii SGB4711 against Philaenus spumarius the main vector of this pathogen, under laboratory conditions. Pathogenicity bioassays were performed by dipping nymphs and adults of P. spumarius in an aqueous suspension of powdered fungal culture (PFC) or conidial suspension (CS) of the three fungal strains.
Both B. bassiana SGB7004 and M. robertsii SGB1K affected the viability of nymphs, resulting in more than 80% mortality at 48 h post treatment, while the effect of A. lecanii SGB4711 was not statistically significant. On adults, all three biocontrol strains were effective in a time- and concentration-dependent manner. The PFCs of B. bassiana SGB7004, M. robertsii SGB1K, and A. lecanii SGB4711 at the highest concentration tested (120 mg mL) resulted in 97%, 83% and 27% mortality at the trial endpoint (120 h), respectively. Mycelial growth was observed on 38.5%, 37.0% and 61.5% of dead insects treated with B. bassiana SGB7004 (2.3 × 10 CFU mL), M. robertsii SGB1K (3.8 × 10 CFU mL) and A. lecanii SGB4711 (5.4 × 10 CFU mL), respectively. None of the PFCs of the tested strains was pathogenic when injected into nymph spittle.
Beauveria bassiana SGB7004 and M. robertsii SGB1K significantly affected the survival of P. spumarius nymphs and adults, while A. lecanii SGB4711 was not effective on nymphs and only slightly effective against adults. © 2024 Society of Chemical Industry.
韧皮部难养菌是意大利普利亚地区橄榄快速衰退综合征的重要病原体。本研究评估了三种昆虫病原真菌菌株的生物功效：球孢白僵菌 SGB7004、金龟子绿僵菌 SGB1K 和蜡蚧轮枝菌 SGB4711，针对该病原体的主要载体斑衣蜡蝉 Philaenus spumarius，在实验室条件下进行。致病性生物测定通过将斑衣蜡蝉的若虫和成虫浸入粉状真菌培养物（PFC）或三种真菌菌株的分生孢子悬浮液（CS）的水溶液中进行。
球孢白僵菌 SGB7004 和金龟子绿僵菌 SGB1K 均影响若虫的生存能力，导致处理后 48 小时死亡率超过 80%，而蜡蚧轮枝菌 SGB4711 的效果则无统计学意义。在成虫方面，三种生防菌株均以时间和浓度依赖的方式有效。在所测试的最高浓度（120mg mL）下，球孢白僵菌 SGB7004、金龟子绿僵菌 SGB1K 和蜡蚧轮枝菌 SGB4711 的 PFC 分别在试验终点（120 小时）导致 97%、83%和 27%的死亡率。用球孢白僵菌 SGB7004（2.3×10 CFU mL）、金龟子绿僵菌 SGB1K（3.8×10 CFU mL）和蜡蚧轮枝菌 SGB4711（5.4×10 CFU mL）处理的 38.5%、37.0%和 61.5%死亡昆虫上观察到菌丝生长。测试菌株的 PFC 均未对注射到若虫唾液中的昆虫产生致病性。
球孢白僵菌 SGB7004 和金龟子绿僵菌 SGB1K 显著影响斑衣蜡蝉若虫和成虫的生存能力，而蜡蚧轮枝菌 SGB4711 对若虫无效，仅对成虫有轻微作用。 © 2024 化学工业协会。
