和的分离、形态特征描述及毒力筛选。

Isolation, morphological characterization, and screening virulence of and fungal isolates n .

机构信息

Ethiopian Institute of Agricultural Research,Ambo Agriculture Research Center, Ambo, Ethiopia.

Ambo University, Ambo, Oromia, 1000, Ethiopia.

出版信息

F1000Res. 2024 Sep 11;12:827. doi: 10.12688/f1000research.134020.2. eCollection 2023.

DOI:10.12688/f1000research.134020.2

PMID:38434644

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10905152/

Abstract

BACKGROUND

Entomopathogenic fungi exists naturally in plants as an asymptote and have the potential to reduce the population of insect pests through indirect interactions. This study was conducted to detect and characterize the endophytic fungi and from the rhizosphere soil of tomato plants and their virulence effect on

METHODS

From the rhizosphere soil of 40 tomato fields, three and seven isolates were isolated using the galleria bait method. All fungi isolate were morphologically characterized by their colony color, shape, and surface texture. Isolates with the highest percentages of germination, conidial yield, and radial growth were selected, and their virulence was evaluated on second instar larvae of under laboratory conditions.

RESULTS

In this study, showed white colony color and aseptate hyphae, whereas Maximum spore production and conidial length were obtained by isolate APPRC-27 with 2.67x10 spores ml and 3.24 µm, respectively. Colony radial growth rates ranged from 1.73 to 3.24 mm day . The results revealed that the highest mortality rate of (100%) was obtained by isolates K-61 and K-102 at a concentration of 1x10 conidial ml at 7 days post-inoculation. The lowest mortality rate was registered by isolate RST-11.

CONCLUSIONS

In the present study, isolates Therefore, virulent entomopathogenic fungi, and , are promising bioagents for the control of insect pests.

摘要

背景

昆虫病原真菌在植物中自然存在，作为一种渐近线，通过间接相互作用有潜力降低害虫种群。本研究旨在检测和描述番茄植物根际土壤中的内生真菌，并研究它们对

方法

采用蕈诱法从 40 个番茄田的根际土壤中分离出三种和七种内生真菌。所有真菌分离物均通过其菌落颜色、形状和表面质地进行形态学特征描述。选择具有最高萌发率、产孢量和径向生长率的分离物，并在实验室条件下评估它们对二龄幼虫的毒力。

结果

在这项研究中，表现出白色菌落颜色和无隔菌丝，而最大孢子产量和分生孢子长度分别由分离物 APPRC-27 获得，为 2.67x10 个孢子 ml 和 3.24 µm。菌落径向生长速率范围为 1.73 至 3.24 mm 天。结果表明，在 7 天接种后，浓度为 1x10 个分生孢子 ml 时，分离物 K-61 和 K-102 对的死亡率最高（100%）。死亡率最低的是分离物 RST-11。