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γ射线辐照诱导磷脂酰胆碱多层脂质体结构改变的X射线衍射和量热法研究

X-ray diffractometry and calorimetry studies of structural modifications induced by gamma-irradiation in phosphatidylcholine multilamellar liposomes.

作者信息

Albertini G, Fanelli E, Guidoni L, Ianzini F, Mariani P, Rustichelli F, Viti V

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Nov;48(5):785-96. doi: 10.1080/09553008514551881.

DOI:10.1080/09553008514551881
PMID:3877016
Abstract

Experimental results are reported on structural and thermodynamic modifications induced by gamma-irradiation in model membranes. Differential scanning calorimetry (DSC) and X-ray diffraction were used to study the different phases and associated transitions of dipalmitoylphosphatidylcholine after 60Co gamma-irradiation. Changes were observed in the shape of calorimetric peaks and in the corresponding enthalpy. The repetition distance of the layers increases while the distances related to the aliphatic chains decrease as a function of gamma-irradiation time. Moreover, an increase in the hexagonal symmetry with increasing dose was detected. No disappearance of a pre-transition was detected even at high doses.

摘要

报告了γ射线辐照在模型膜中引起的结构和热力学变化的实验结果。采用差示扫描量热法(DSC)和X射线衍射研究了60Coγ射线辐照后二棕榈酰磷脂酰胆碱的不同相及相关转变。观察到量热峰形状和相应焓的变化。随着γ射线辐照时间的增加,层的重复距离增加,而与脂肪链相关的距离减小。此外,检测到随着剂量增加六方对称性增加。即使在高剂量下也未检测到预转变的消失。

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