Ianzini F, Guidoni L, Indovina P L, Viti V, Erriu G, Onnis S, Randaccio P
Radiat Res. 1984 Apr;98(1):154-66.
Chemical and structural modifications in multilayer liposomes of synthetic phosphatidylcholine induced by gamma irradiation are investigated with different techniques. Fluorescence anisotropy of the DPH probe and differential scanning calorimetry reveal a broadening of the main lipid transition and the disappearance of pretransition. Fluorescence anisotropy is shown to be higher in the irradiated sample and particularly so at low temperatures. NMR and TLC results show that lysolecithin and palmitic acid are formed with a consequent change in bilayer organization. The possibility that these modifications may account for the permeability variations observed in irradiated natural membranes is discussed.
采用不同技术研究了γ射线辐照诱导的合成磷脂酰胆碱多层脂质体的化学和结构修饰。DPH探针的荧光各向异性和差示扫描量热法显示主要脂质转变变宽且预转变消失。辐照样品中的荧光各向异性较高，尤其是在低温下。核磁共振和薄层色谱结果表明形成了溶血卵磷脂和棕榈酸，从而导致双层结构发生变化。讨论了这些修饰可能解释辐照天然膜中观察到的通透性变化的可能性。
