Department of Respiratory Medicine, First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China; Department of Respiratory Medicine, Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.
Department of Respiratory Medicine, Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China.
Comput Biol Med. 2024 Jul;177:108574. doi: 10.1016/j.compbiomed.2024.108574. Epub 2024 May 11.
The immune dysregulation associated with carbapenem-resistant Klebsiella pneumoniae (CRKP) severity was investigated through single-cell RNA sequencing (scRNA-seq) of 5 peripheral blood samples from 3 patients with moderate and severe CRKP pneumonia. Additionally, scRNA-seq datasets from two individuals with COVID-19 were included for comparative analysis. The dynamic characterization and functional properties of each immune cell type were examined by delineating the transcriptional profiles of immune cells throughout the transition from moderate to severe conditions. Overall, most immune cells in CRKP patients exhibited a robust interferon-α response and inflammatory reaction compared to healthy controls, mirroring observations in COVID-19 patients. Furthermore, cell signatures associated with NK cells, macrophages, and monocytes were identified in CRKP progression including PTPRCAP for NK cells, C1QB for macrophages, and S100A12 for both macrophages and monocytes. In summary, this study offers a comprehensive scRNA-seq resource for illustrating the dynamic immune response patterns during CRKP progression, thereby shedding light on the associations between CRKP and COVID-19.
通过对 3 名中重度耐碳青霉烯类肺炎克雷伯菌（CRKP）肺炎患者的 5 份外周血样本进行单细胞 RNA 测序（scRNA-seq），研究了与 CRKP 严重程度相关的免疫失调。此外，还纳入了两名 COVID-19 患者的 scRNA-seq 数据集进行比较分析。通过描绘从中度到重度疾病转变过程中免疫细胞的转录谱，对每种免疫细胞类型的动态特征和功能特性进行了检查。总体而言，与健康对照组相比，CRKP 患者的大多数免疫细胞表现出强烈的干扰素-α反应和炎症反应，这与 COVID-19 患者的观察结果相似。此外，在 CRKP 进展过程中确定了与 NK 细胞、巨噬细胞和单核细胞相关的细胞特征，包括 NK 细胞的 PTPRCAP、巨噬细胞的 C1QB 和巨噬细胞和单核细胞的 S100A12。总之，这项研究提供了一个全面的 scRNA-seq 资源，用于说明 CRKP 进展过程中的动态免疫反应模式，从而揭示了 CRKP 与 COVID-19 之间的关联。
