College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety; Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, P.R. China.
National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Int J Food Microbiol. 2024 Jul 16;419:110747. doi: 10.1016/j.ijfoodmicro.2024.110747. Epub 2024 May 15.
Campylobacter jejuni is recognized as a significant foodborne pathogen, and recent studies have indicated a rising trend of aminoglycosides resistance gene aph(2″)-If among C. jejuni isolates from food-producing animals in China. However, systematic information about aph(2″)-If-positive C. jejuni from food-producing animals and other sources worldwide based on whole-genome analysis remains a knowledge gap. In this study, we aimed to analyze the worldwide distribution, genetic environment and phylogenetic tree of aph(2″)-If by utilizing Whole Genome Sequencing (WGS) data obtained, coupled with information in the GenBank database. A total of 160C. jejuni isolates in the GenBank database and 14C. jejuni isolates in our laboratory carrying aph(2″)-If gene were performed for further analysis. WGS analysis revealed the global distribution of aph(2″)-If among C. jejuni from 6 countries. Multilocus Sequence Typing(MLST) results indicated that 70 STs were involved in the dissemination of aph(2″)-If, with ST10086 being the predominant ST. Whole-genome Multilocus Sequence Typing(wg-MLST) analysis according to times, countries, and origins of C. jejuni isolation further demonstrated a close relationship between aph(2″)-If carrying C. jejuni isolates from farm and food. The findings also revealed the existence of 32 distinct types of genetic environments surrounding aph(2″)-If among these isolates. Notably, Type 30, characterized by the arrangement ISsag10-deoD-ant(9)-hp-hp-aph(2″)-If, emerged as the predominant genetic environment. In conclusion, our analysis provides the inaugural perspective on the worldwide distribution of aph(2″)-If. This resistance gene demonstrates horizontal transferability and regional diffusion in a clonal pattern. The close association observed among aph(2″)-If-positive C. jejuni strains isolated from poultry, food, and clinical environments underscores the potential for zoonotic transmission from these isolates.
空肠弯曲菌被认为是一种重要的食源性致病菌，最近的研究表明，中国食源性动物源空肠弯曲菌分离株中氨基糖苷类耐药基因 aph(2″)-If 的出现呈上升趋势。然而，基于全基因组分析的食源性动物和其他来源的 aph(2″)-If 阳性空肠弯曲菌的全球系统信息仍然是一个知识空白。在本研究中，我们旨在通过利用全基因组测序 (WGS) 数据和 GenBank 数据库中的信息，分析 aph(2″)-If 在全球的分布、遗传环境和系统发育树。对 GenBank 数据库中的 160 株空肠弯曲菌分离株和实验室中的 14 株携带 aph(2″)-If 基因的空肠弯曲菌分离株进行了进一步分析。WGS 分析揭示了 aph(2″)-If 在来自 6 个国家的空肠弯曲菌中的全球分布。多位点序列分型 (MLST) 结果表明，70 个 ST 参与了 aph(2″)-If 的传播，其中 ST10086 是主要的 ST。根据时间、国家和空肠弯曲菌分离源进行的全基因组多位点序列分型 (wg-MLST) 分析进一步表明，携带 aph(2″)-If 的空肠弯曲菌分离株来自农场和食品之间存在密切关系。研究结果还表明，在这些分离株中，aph(2″)-If 周围存在 32 种不同类型的遗传环境。值得注意的是，以 ISsag10-deoD-ant(9)-hp-hp-aph(2″)-If 排列为特征的 Type 30 是主要的遗传环境。总之，我们的分析提供了 aph(2″)-If 全球分布的初步视角。该耐药基因表现出水平可转移性和以克隆模式的区域扩散。在禽、食品和临床环境中分离的 aph(2″)-If 阳性空肠弯曲菌菌株之间的密切关联表明，这些分离株可能具有从动物传播给人类的潜力。
