McMahon M S, Norregaard T V, Beyerl B D, Borges L F, Moskowitz M A
Neurosci Lett. 1985 Sep 16;60(1):63-8. doi: 10.1016/0304-3940(85)90382-9.
Horseradish peroxidase conjugated to wheat germ agglutinin (HRP-WGA), and bisbenzimide (a fluorescent dye) were used as retrograde axonal tracers to examine whether or not intracranial and extracranial trigeminal afferents represent divergent axon collaterals. HRP-WGA was applied to the proximal segment of the middle cerebral artery and bisbenzimide was injected into a branch of the ophthalmic nerve in 5 cats. Histologic examination of the ipsilateral trigeminal ganglion revealed HRP-labeled cell bodies located among clusters of cells exhibiting bisbenzimide fluorescence. Cells containing both labels were not observed. These results support the concept that divergent axon collaterals are probably not involved in the pathogenesis of referred pain during vascular headache.
将与小麦胚凝集素结合的辣根过氧化物酶（HRP-WGA）和双苯甲酰亚胺（一种荧光染料）用作逆行轴突示踪剂，以检查颅内和颅外三叉神经传入纤维是否代表不同的轴突侧支。在5只猫中，将HRP-WGA应用于大脑中动脉的近端段，并将双苯甲酰亚胺注入眼神经的一个分支。对同侧三叉神经节的组织学检查显示，HRP标记的细胞体位于呈现双苯甲酰亚胺荧光的细胞簇中。未观察到含有两种标记的细胞。这些结果支持这样一种概念，即不同的轴突侧支可能不参与血管性头痛期间牵涉痛的发病机制。
