Partridge N C, Hillyard C J, Nolan R D, Martin T J
Prostaglandins. 1985 Sep;30(3):527-39. doi: 10.1016/0090-6980(85)90124-8.
The effect of various factors upon prostaglandin (PG) production by the osteoblast was examined using osteoblast-rich populations of cells prepared from newborn rat calvaria. Bradykinin and serum, and to a lesser extent, thrombin, were all shown to stimulate PGE2 and 6-keto-PGF1 alpha (the hydration product of PGI2) secretion by the osteoblastic cells. Several inhibitors of prostanoid synthesis, dexamethasone, indomethacin, dazoxiben and nafazatrom, were tested for their effects on the calvarial cells. All inhibited PGE2 and PGI2 (the major arachidonic acid metabolites of these cells) production with half-maximal inhibition by all four substances occurring at approximately 10(-7) M. For dazoxiben and nafazatrom, this was in contrast to published results from experiments in vivo which have indicated that the compounds stimulated PGI2 production. Finally, since the osteoblast is responsive to bone-resorbing hormones, these were tested. Only epidermal growth factor (EGF) was shown to modify PG production. At early times EGF stimulated PGE2 release, however, the predominant effect of the growth factor was an inhibition of both PGE2 and PGI2 production by the osteoblastic cells. The present results suggest that the bone-resorbing hormones do not act to cause an increase in PG by the osteoblast and that any increase in PG production by these cells may be in response to vascular agents.
利用从新生大鼠颅骨制备的富含成骨细胞的细胞群体，研究了各种因素对成骨细胞前列腺素（PG）产生的影响。缓激肽和血清，以及程度较轻的凝血酶，均显示可刺激成骨细胞分泌前列腺素E2（PGE2）和6-酮-前列腺素F1α（前列环素I2（PGI2）的水化产物）。测试了几种前列腺素合成抑制剂，地塞米松、吲哚美辛、达唑氧苯和萘呋胺酯，观察它们对颅骨细胞的影响。所有这些抑制剂均抑制PGE2和PGI2（这些细胞主要的花生四烯酸代谢产物）的产生，所有四种物质的半数最大抑制浓度约为10^(-7)M。对于达唑氧苯和萘呋胺酯，这与体内实验已发表的结果相反，体内实验表明这些化合物可刺激PGI2的产生。最后，由于成骨细胞对骨吸收激素有反应，因此对这些激素进行了测试。仅发现表皮生长因子（EGF）可改变PG的产生。在早期，EGF刺激PGE2释放，然而，该生长因子的主要作用是抑制成骨细胞产生PGE2和PGI2。目前的结果表明，骨吸收激素不会促使成骨细胞增加PG的产生，并且这些细胞PG产生的任何增加可能是对血管活性物质的反应。
