Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, Olsztyn, 10-719, Poland.
Faculty of Medical Sciences, University of Warmia and Mazury in Olsztyn, Aleja Warszawska 30, Olsztyn, 10-082, Poland.
BMC Genomics. 2024 May 21;25(1):501. doi: 10.1186/s12864-024-10417-9.
The peri-implantation period is a critical time during pregnancy that mostly defines the overall litter size. Most authors agree that the highest percentage of embryo mortality occurs during this time. Despite the brevity of the peri-implantation period, it is the most dynamic part of pregnancy in which the sequential and uninterrupted course of several processes is essential to the animal's reproductive success. Also then, the maternal uterine tissues undergo an intensive remodelling process, and their energy demand dramatically increases. It is believed that apelin, a member of the adipokine family, is involved in the control of female reproductive functions in response to the current metabolic state. The verified herein hypothesis assumed the modulatory effect of apelin on the endometrial tissue transcriptome on days 15 to 16 of gestation (beginning of implantation).
The analysis of data obtained during RNA-seq (Illumina HiSeq2500) of endometrial slices treated and untreated with apelin (n = 4 per group) revealed changes in the expression of 68 genes (39 up-regulated and 29 down-regulated in the presence of apelin), assigned to 240 gene ontology terms. We also revealed changes in the frequency of alternative splicing events (397 cases), as well as single nucleotide variants (1,818 cases) in the presence of the adipokine. The identified genes were associated, among others, with the composition of the extracellular matrix, apoptosis, and angiogenesis.
The obtained results indicate a potential role of apelin in the regulation of uterine tissue remodelling during the peri-implantation period.
胚胎着床期是妊娠的关键时期，它主要决定了整体胎儿数量。大多数作者都认为胚胎死亡率在这段时间内最高。尽管胚胎着床期很短，但它是妊娠中最具活力的部分，其中几个过程的连续和不间断过程对动物的生殖成功至关重要。此时，母体子宫组织也经历了一个密集的重塑过程，其能量需求急剧增加。人们认为，apelin 是脂肪因子家族的一员，它参与了对当前代谢状态的雌性生殖功能的控制。本文假设 apelin 对妊娠第 15 至 16 天（着床开始时）子宫内膜组织转录组有调节作用。
对用和未用 apelin 处理的子宫内膜切片进行 RNA-seq（Illumina HiSeq2500）分析获得的数据进行分析，结果显示 68 个基因的表达发生了变化（apelin 存在时 39 个上调，29 个下调），这些基因被分配到 240 个基因本体术语中。我们还发现了在存在脂肪因子时，剪接事件（397 例）和单核苷酸变异（1818 例）的频率发生了变化。所鉴定的基因与细胞外基质组成、细胞凋亡和血管生成等有关。
这些结果表明 apelin 在胚胎着床期子宫组织重塑的调节中具有潜在作用。
