Pato M L
J Bacteriol. 1979 Nov;140(2):518-24. doi: 10.1128/jb.140.2.518-524.1979.
Inhibition of ribonucleic acid synthesis in Escherichia coli 15 TAU bar with rifampin or streptolydigin leads to large increases in the sizes of cellular ribonucleoside and deoxyribonucleoside triphosphate pools. Inhibition of protein synthesis leads to increases in the sizes of all nucleoside triphosphate pools except the guanosine triphosphate and deoxyguanosine triphosphate pools; a decrease in the size of the latter pool may be responsible for the slowing of deoxyribonucleic acid replication fork movement observed in this strain in the absence of protein synthesis. Analysis of the kinetics of incorporation of labeled precursors into deoxyribonucleic acid and into cellular pools suggests that functional compartmentation of nucleotide pools exists, allowing the incorporation of exogenously supplied precursors into deoxyribonucleic acid without prior equilibration with the cellular pools.
用利福平或链霉溶菌素抑制大肠杆菌15 TAU bar中的核糖核酸合成会导致细胞核糖核苷和脱氧核糖核苷三磷酸池的大小大幅增加。蛋白质合成的抑制会导致除鸟苷三磷酸和脱氧鸟苷三磷酸池外的所有核苷三磷酸池大小增加；后一种池大小的减少可能是导致在该菌株中缺乏蛋白质合成时观察到的脱氧核糖核酸复制叉移动减慢的原因。对标记前体掺入脱氧核糖核酸和细胞池的动力学分析表明，核苷酸池存在功能区室化，使得外源供应的前体无需先与细胞池平衡就能掺入脱氧核糖核酸中。
