Nyberg G K, Carlsson J
Antimicrob Agents Chemother. 1981 Dec;20(6):726-30. doi: 10.1128/AAC.20.6.726.
Peptostreptococcus anaerobius VPI 4330-1 was tested under various conditions for examination of the bactericidal effect of hydrogen peroxide. The cells were most rapidly killed by hydrogen peroxide when they were in the exponential-growth phase. Cooling or starving the cells decreased the bactericidal effect of hydrogen peroxide. The ionophore nigericin and the metal ion chelating agent 2,2'-bipyridine stopped macromolecular syntheses and greatly decreased the bactericidal effect of hydrogen peroxide. The ionophore valinomycin and the glycolytic inhibitor iodoacetate also stopped the syntheses of the macromolecules but only slightly decreased the bactericidal effect of hydrogen peroxide. Novobiocin, an inhibitor of deoxyribonucleic acid gyrase, and chloramphenicol, an inhibitor of protein synthesis, were not able to decrease the bactericidal effect of hydrogen peroxide. These findings implicate metal ions and an active metabolism of the organism in the bactericidal effect of hydrogen peroxide.
对厌氧消化链球菌VPI 4330-1在各种条件下进行测试，以检测过氧化氢的杀菌效果。处于指数生长期的细胞被过氧化氢杀死的速度最快。细胞冷却或饥饿会降低过氧化氢的杀菌效果。离子载体尼日利亚菌素和金属离子螯合剂2,2'-联吡啶会停止大分子合成，并大大降低过氧化氢的杀菌效果。离子载体缬氨霉素和糖酵解抑制剂碘乙酸盐也会停止大分子合成，但只会略微降低过氧化氢的杀菌效果。脱氧核糖核酸回旋酶抑制剂新生霉素和蛋白质合成抑制剂氯霉素无法降低过氧化氢的杀菌效果。这些发现表明金属离子和生物体的活跃代谢在过氧化氢的杀菌效果中起作用。
