Mohammed Rebar N, Khoshnaw Najmaddin S H, Mohammed Vian Faeq, Hassan Dastan O, Abdullah Chra Nawfal, Mahmood Tavan Ismael, Abbass Huda A, Ahmed Dereen, Noori Kani D, Saeed Lanja I, Salih Salah Mohammed, Sidiq Hiwa S, Ali Dlnya Omer, Shwan Alan, Majolino Ignazio, Ipsevich Francesco
Department of Medical Laboratory Technology, Faculty of Health Science, Qaiwan International University, Sulaimani, Kurdistan Region, Iraq.
College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Iraq.
Heliyon. 2024 May 8;10(9):e30888. doi: 10.1016/j.heliyon.2024.e30888. eCollection 2024 May 15.
Hematopoietic stem cell transplantation is still a curative treatment for many haematological cancers. Many factors, such as age, sex, ethnic background, smoking status, and body mass index, affect average reference values in different populations. This study aimed to establish a reference range for the absolute numbers and percentages of healthy individuals' hematopoietic stem cells and immune cells in the bone marrow. Seventy-one healthy donors (32 males and 39 females) were enrolled in the study. Following bone marrow harvesting, using flow cytometry, immunophenotyping was performed to determine the absolute number and percentage of CD34 stem cells and various immune subsets. We found no statistically significant difference in the absolute count of HSCs or immune cell subsets in the bone marrow between males and females. Regarding age, the younger group had more significant CD34 and immune cell subsets. Donors with healthier body weights tend to have richer bone marrow cellularity. Establishing a reference value for hematopoietic stem cells and immune cells in the bone marrow based on various influential factors is pivotal for defining bone marrow status and donor selection.
造血干细胞移植仍然是许多血液系统癌症的一种治愈性治疗方法。许多因素，如年龄、性别、种族背景、吸烟状况和体重指数，会影响不同人群的平均参考值。本研究旨在建立健康个体骨髓中造血干细胞和免疫细胞绝对数量及百分比的参考范围。71名健康供者（32名男性和39名女性）参与了该研究。在采集骨髓后，使用流式细胞术进行免疫表型分析，以确定CD34干细胞和各种免疫亚群的绝对数量和百分比。我们发现男性和女性骨髓中造血干细胞或免疫细胞亚群的绝对计数没有统计学上的显著差异。关于年龄，较年轻的组有更显著的CD34和免疫细胞亚群。体重更健康的供者往往骨髓细胞含量更丰富。基于各种影响因素建立骨髓造血干细胞和免疫细胞的参考值对于定义骨髓状态和供者选择至关重要。
