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一个具有改变启动子序列的合成酪氨酸抑制tRNA基因。其克隆及在体内的相对表达。

A synthetic tyrosine suppressor tRNA gene with an altered promoter sequence. Its cloning and relative expression in vivo.

作者信息

Ryan M J, Belagaje R, Brown E L, Fritz H J, Khorana H G

出版信息

J Biol Chem. 1979 Nov 10;254(21):10803-10.

PMID:387755
Abstract

The total synthesis of a tyrosine suppressor tRNA gene with a modified promoter is described. The alteration involves the replacement of the four G:C base pairs immediately preceding the start point of transcription by A:T base pairs. The new sequence contains the recognition sequence for the HindIII restriction endonuclease at the transcriptional start point, thus permitting fusion of the structural gene with promoters containing independent sequence modifications. The construction, cloning, and biological activity of several recombinant DNAs containing the tRNA gene with the modified promoter are described. The expression of this gene in vivo is compared with that of both the unmodified synthetic suppressor gene and a naturally occurring tyr su3+ gene cloned onto a multicopy plasmid.

摘要

描述了具有修饰启动子的酪氨酸抑制性tRNA基因的全合成。这种改变涉及将转录起始点之前紧邻的四个G:C碱基对替换为A:T碱基对。新序列在转录起始点处包含HindIII限制性内切核酸酶的识别序列，从而允许结构基因与包含独立序列修饰的启动子融合。描述了几种含有修饰启动子的tRNA基因的重组DNA的构建、克隆和生物活性。将该基因在体内的表达与未修饰的合成抑制基因以及克隆到多拷贝质粒上的天然存在的tyr su3+基因的表达进行了比较。

相似文献

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A synthetic tyrosine suppressor tRNA gene with an altered promoter sequence. Its cloning and relative expression in vivo.一个具有改变启动子序列的合成酪氨酸抑制tRNA基因。其克隆及在体内的相对表达。
J Biol Chem. 1979 Nov 10;254(21):10803-10.
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Total synthesis of a tyrosine suppressor tRNA gene. XVIII. Biological activity and transcription, in vitro, of the cloned gene.酪氨酸抑制性tRNA基因的全合成。十八。克隆基因的生物活性及体外转录。
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The synthesis and cloning of two tyrosine suppressor tRNA genes with altered promoter sequences.两个具有改变启动子序列的酪氨酸抑制性tRNA基因的合成与克隆。
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EMBO J. 1993 Jun;12(6):2483-94. doi: 10.1002/j.1460-2075.1993.tb05903.x.
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Nucleic Acids Res. 1980 Nov 25;8(22):5445-59. doi: 10.1093/nar/8.22.5445.
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一个在非洲爪蟾卵母细胞中无活性的原核tRNATyr基因，通过与真核tRNAPro基因重组而被激活。
EMBO J. 1982;1(7):817-20. doi: 10.1002/j.1460-2075.1982.tb01253.x.
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PpGpp regulates the binding of two RNA polymerase molecules to the tyrT promoter.鸟苷四磷酸（ppGpp）调节两个RNA聚合酶分子与tyrT启动子的结合。
Nucleic Acids Res. 1982 Aug 25;10(16):5043-57. doi: 10.1093/nar/10.16.5043.
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Nucleic Acids Res. 1988 Aug 25;16(16):7763-71. doi: 10.1093/nar/16.16.7763.
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