Hospital Israelita Albert Einstein, São Paulo, SP, Brazil.
LIM44 - Hospital das Clínicas, Faculdade Medicina, Universidade de São Paulo, São Paulo, SP, Brazil.
Einstein (Sao Paulo). 2024 May 20;22:eAO0764. doi: 10.31744/einstein_journal/2024AO0764. eCollection 2024.
To evaluate the in vitro and in vivo toxicities of polyethylene glycol-coated gold nanoparticles synthesized using a one-step process.
Gold nanoparticles were prepared via a co-precipitation method using polyethylene glycol, and the synthesis product was characterized. For the in vitro evaluation, a flow cytometry analysis with Annexin V and iodide propidium staining was used to assess cytotoxicity in MG-63 cells labeled with 10, 50, and 100µg/mL of nanoparticle concentration. For the in vivo evaluation, nanoparticles were administered intraperitoneally at a dose of 10mg/kg dose in 10-week-old mice. Toxicity was assessed 24 hours and 7 days after administration via histopathological analysis of various tissues, as well as through renal, hepatic, and hematopoietic evaluations.
Synthesized nanoparticles exhibited different hydrodynamic sizes depending on the medium: 51.27±1.62nm in water and 268.12±28.45nm (0 hour) in culture medium. They demonstrated a maximum absorbance at 520nm and a zeta potential of -8.419mV. Cellular viability exceeded 90%, with less than 3% early apoptosis, 6% late apoptosis, and 1% necrosis across all labeling conditions, indicating minimal cytotoxicity differences. Histopathological analysis highlighted the accumulation of nanoparticles in the mesentery; however, no lesions or visible agglomeration was observed in the remaining tissues. Renal, hepatic, and hematopoietic analyses showed no significant differences at any time point.
Polyethylene glycol-coated gold nanoparticles exhibit extremely low toxicity and high biocompatibility, showing promise for future studies.
评估一步法合成的聚乙二醇包覆金纳米粒子的体外和体内毒性。
采用聚乙二醇共沉淀法制备金纳米粒子，并对合成产物进行了表征。体外评估采用碘化丙啶和 Annexin V 流式细胞术分析，评估浓度为 10、50 和 100μg/mL 的纳米粒子标记的 MG-63 细胞的细胞毒性。体内评估采用腹腔内给药，剂量为 10mg/kg，在 10 周龄小鼠中进行。给药后 24 小时和 7 天，通过对各种组织进行组织病理学分析，以及对肾脏、肝脏和造血系统进行评估，来评估毒性。
合成的纳米粒子在不同介质中表现出不同的水动力粒径：水中为 51.27±1.62nm，培养基中为 268.12±28.45nm（0 小时）。它们在 520nm 处表现出最大吸收峰，zeta 电位为-8.419mV。细胞活力均超过 90%，各标记条件下早期凋亡率小于 3%，晚期凋亡率 6%，坏死率 1%，表明细胞毒性差异极小。组织病理学分析突出了纳米粒子在肠系膜中的积累，但在其余组织中未观察到病变或明显聚集。肾、肝和造血分析在任何时间点均未显示出显著差异。
聚乙二醇包覆金纳米粒子表现出极低的毒性和高度的生物相容性，有望用于未来的研究。
