Saavedra R A, Cordoba C, Anderson G R
Exp Eye Res. 1985 Sep;41(3):365-70. doi: 10.1016/s0014-4835(85)80027-0.
LDHk is a cancer-associated lactate dehydrogenase which is also found at high levels in normal mammalian retina. Such retinas share with most cancer tissues a dependence on aerobic glycolysis, leading to high production of lactate. However, retinas of lower vertebrate species are significantly less dependent on aerobic glycolysis. We find that retinas of species less dependent on aerobic glycolysis express significantly lower levels of an LDHk-like activity, less than or equal to the low levels seen in brains. The enzymes from lower species differ from the mammalian retinal enzyme in their pH optima and responsiveness to oxygen; but share a similar degree of inhibition by 5'-5'-dinucleoside tetraphosphates. Therefore, the expression pattern of LDHk in brain and retina of diverse vertebrate species suggests a link with the Warburg effect.
乳酸脱氢酶k是一种与癌症相关的乳酸脱氢酶，在正常哺乳动物视网膜中也大量存在。此类视网膜与大多数癌组织一样依赖有氧糖酵解，导致乳酸大量生成。然而，低等脊椎动物物种的视网膜对有氧糖酵解的依赖程度要低得多。我们发现，对有氧糖酵解依赖程度较低的物种的视网膜中，类似乳酸脱氢酶k的活性水平显著更低，小于或等于在大脑中所见的低水平。来自低等物种的酶在最适pH值和对氧气的反应性方面与哺乳动物视网膜酶不同；但对5'-5'-二核苷四磷酸的抑制程度相似。因此，不同脊椎动物物种大脑和视网膜中乳酸脱氢酶k的表达模式表明其与瓦伯格效应存在联系。
