Galelli A, Dosne A M, Morin A, Dubor F, Chedid L
Exp Hematol. 1985 Dec;13(11):1157-63.
The in vivo induction of colony-stimulating activity (CSA) by well-defined immunomodulatory synthetic muramyl peptides has been demonstrated recently in mice. In the present study, we tested the capacities of three muramyl peptides to induce CSA production in human endothelial cell (HEC) cultures. Two adjuvant-active peptides (MDP and Murabutide) induced CSA in the supernatant of cultured endothelial cells, whereas an adjuvant-inactive compound had no effect. This effect of MDP and Murabutide appeared to be time and concentration dependent and was not secondary to decreased production of inhibitors of colony formation. CSA secretion by stimulated HEC required de novo protein synthesis and did not result from the release of preformed active CSA. Maximal concentration appeared in the supernatant media within the first 24 h after addition of muramyl peptides, and a substantial second CSA secretion could be observed after a subsequent 24 h reexposure. This CAS was not dialyzable and promoted granulocyte-macrophage formation of nonadherent human marrow and unfractionated murine marrow. Our data demonstrate that the human endothelial cell is a target cell for MDP and Murabutide and suggest that in vivo endothelium might play an active role in muramyl peptide-induced modulation of hematopoiesis.
最近在小鼠中已证实，明确的免疫调节合成胞壁酰肽可在体内诱导集落刺激活性（CSA）。在本研究中，我们测试了三种胞壁酰肽在人内皮细胞（HEC）培养物中诱导CSA产生的能力。两种具有佐剂活性的肽（MDP和Murabutide）可在培养的内皮细胞上清液中诱导CSA，而一种无佐剂活性的化合物则无作用。MDP和Murabutide的这种作用似乎具有时间和浓度依赖性，并非是集落形成抑制剂产生减少的继发效应。受刺激的HEC分泌CSA需要重新合成蛋白质，并非预先形成的活性CSA释放所致。添加胞壁酰肽后的最初24小时内，上清液培养基中出现最大浓度，随后再次暴露24小时后可观察到大量的第二次CSA分泌。这种CSA不可透析，并促进非贴壁人骨髓和未分级小鼠骨髓的粒细胞-巨噬细胞形成。我们的数据表明，人内皮细胞是MDP和Murabutide的靶细胞，并提示体内内皮细胞可能在胞壁酰肽诱导的造血调节中发挥积极作用。
