Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and iChem, Fudan University, Shanghai 200433, China.
Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials and iChem, Fudan University, Shanghai 200433, China.
Curr Opin Chem Biol. 2024 Jun;80:102469. doi: 10.1016/j.cbpa.2024.102469. Epub 2024 May 21.
In vivo luminescence imaging in the second near-infrared window (NIR-II, 1000-2000 nm) is a potent technique for observing deep-tissue life activities, leveraging reduced light scattering, minimized autofluorescence, and moderate absorption attenuation to substantially enhance image contrast. Pushing the frontiers of NIR-II luminescence imaging forward, moving from static to dynamic event visualization, monochromatic to multicolor images, and fundamental research to clinical applications, necessitates the development of novel luminophores featuring bright emission, extendable wavelength, and optimal biocompatibility. Recently, lanthanide-dye hybrid luminophores (LDHLs) are gaining increasing attention for their wavelength extensibility, molecular size, narrowband emission, mega stokes shift, long lifetime, and high photostability. In this review, we will summarize the recent advances of NIR-II LDHLs and their applications in imaging and analysis of living mammals, and discuss future challenges in designing new LDHLs for deep-tissue imaging.
体内近红外二区（NIR-II，1000-2000nm）的生物发光成像是一种强大的技术，用于观察深层组织的生命活动，利用减少的光散射、最小化的自发荧光和适度的吸收衰减，大大提高了图像对比度。为了推动 NIR-II 生物发光成像的前沿发展，从静态到动态事件可视化、从单色到多色图像，以及从基础研究到临床应用，需要开发具有明亮发射、可扩展波长和最佳生物相容性的新型发光体。最近，镧系染料混合发光体（LDHLs）因其波长可扩展性、分子尺寸、窄带发射、超大斯托克斯位移、长寿命和高光稳定性而受到越来越多的关注。在这篇综述中，我们将总结 NIR-II LDHLs 的最新进展及其在活体哺乳动物成像和分析中的应用，并讨论为深层组织成像设计新型 LDHLs 所面临的未来挑战。
