Nautiyal Abhilek K, Paul Pranoy, Raut Sachin, Bhaskar Thallada, Chowdhury Nilotpal, Khatri Neeraj, Ghosh Debashish
Material Resource Efficiency Division, CSIR-Indian Institute of Petroleum, Mohkampur, Dehradun, Uttarakhand, 248005, India.
IMTech Centre for Animal Resources & Experimentation (iCARE), CSIR-Institute of Microbial Technology (IMTECH), Chandigarh, 160036, India.
World J Microbiol Biotechnol. 2024 May 23;40(7):211. doi: 10.1007/s11274-024-03976-2.
Human nutrition and health rely on edible oils. Global demand for edible oils is expanding, necessitating the discovery of new natural oil sources subjected to adequate quality and safety evaluation. However, in contrast to other agricultural products, India's edible oil supply is surprisingly dependent on imports. The microbial oil is generated by fermentation of oleaginous yeast Rhodotorula mucilaginosa IIPL32 MTCC 25056 using biodiesel plant byproduct crude glycerol as a fermentable carbon source. Enriched with monounsaturated fatty acid, nutritional indices mapping based on the fatty acid composition of the yeast SCO, suggested its plausible use as an edible oil blend. In the present study, acute toxicity evaluation of the yeast SCO in C57BL/6 mice has been performed by randomly dividing the animals into 5 groups with 50, 300, 2000, and 5000 mg/Kg yeast SCO dosage, respectively, and predicted the median lethal dose (LD50). Detailed blood biochemistry and kidney and liver histopathology analyses were also reported. The functions of the liver enzymes were also evaluated to check and confirm the anticipated toxicity. To determine cell viability and in vitro biocompatibility, the 3T3-L1 cell line and haemolysis tests were performed. The results suggested the plausible use of yeast SCO as an edible oil blend due to its non-toxic nature in mice models.
人类营养与健康依赖于食用油。全球对食用油的需求不断扩大，因此需要发现新的天然油源并对其进行充分的质量和安全评估。然而，与其他农产品不同的是，印度的食用油供应惊人地依赖进口。微生物油是由产油酵母黏液红酵母IIPL32 MTCC 25056以生物柴油厂副产品粗甘油作为可发酵碳源发酵产生的。富含单不饱和脂肪酸，基于酵母单细胞油脂肪酸组成的营养指标图谱表明其有望用作食用油混合物。在本研究中，通过将C57BL/6小鼠随机分为5组，分别给予50、300、2000和5000 mg/Kg酵母单细胞油剂量，对酵母单细胞油进行了急性毒性评估，并预测了半数致死剂量（LD50）。还报告了详细的血液生化以及肾脏和肝脏组织病理学分析。还评估了肝酶的功能以检查和确认预期的毒性。为了确定细胞活力和体外生物相容性，进行了3T3-L1细胞系和溶血试验。结果表明，由于酵母单细胞油在小鼠模型中无毒性质，其有望用作食用油混合物。
