Lorca-Arce Daniel, Pérez-Isidro Albert, Becerra Judit, Martínez Maria José, De Moner Noemí, Ríos-Garcés Roberto, Prieto-González Sergio, Espinosa Gerard, Cervera Ricard, Andalucía Carmen, Viñas-Gomis Odette, Ruiz-Ortiz Estibaliz
Immunology Department, Centre Diagnostic Biomèdic CDB, Hospital Clínic de Barcelona, Barcelona, Spain.
Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain.
Heliyon. 2024 May 9;10(10):e30767. doi: 10.1016/j.heliyon.2024.e30767. eCollection 2024 May 30.
Anti-dsDNA, anti-Sm, and anti-ribosomal-P autoantibodies are hallmarks of systemic lupus erythematosus (SLE), being anti-dsDNA and anti-Sm included in 2019-ACR/EULAR SLE-Classification Criteria. Enzyme-linked (ELISA) and chemiluminescence assays (CIA) are widely established in immunology laboratories, but new technologies, such as particle-based multi-analyte technology (PMAT), are nowadays available. The present study aimed to compare the presence of anti-dsDNA and anti-Sm autoantibodies measured by CIA and PMAT and analyze diagnostic and clinical SLE activity performance. Anti-ribosomal-P autoantibodies by PMAT were also included. Consequently, anti-dsDNA and anti-Sm detected by CIA showed substantial agreement with PMAT (Cohen's = 0.662 and 0.671, respectively). Anti-dsDNA autoantibodies measured by PMAT showed a positive correlation with clinical SLEDAI-2K ( < 0.001) and a negative correlation with complement consumption ( < 0.001). Anti-Sm and anti-ribosomal-P autoantibodies showed a positive correlation with SLEDAI-2K ( < 0.001 and = 0.001, respectively) and a negative correlation with complement consumption ( < 0.001 and = 0.001, respectively). Finally, anti-Sm autoantibodies were associated with renal involvement ( < 0.05).
抗双链DNA、抗Sm和抗核糖体P自身抗体是系统性红斑狼疮（SLE）的标志，抗双链DNA和抗Sm被纳入2019年美国风湿病学会/欧洲抗风湿病联盟（ACR/EULAR）SLE分类标准。酶联免疫吸附测定（ELISA）和化学发光测定（CIA）在免疫实验室中广泛应用，但如今也有基于颗粒的多分析物技术（PMAT）等新技术。本研究旨在比较用CIA和PMAT检测的抗双链DNA和抗Sm自身抗体的存在情况，并分析SLE的诊断及临床活动表现。PMAT检测的抗核糖体P自身抗体也被纳入研究。结果显示，CIA检测的抗双链DNA和抗Sm与PMAT检测结果有高度一致性（Cohen's值分别为0.662和0.671）。PMAT检测的抗双链DNA自身抗体与临床SLE疾病活动指数2000（SLEDAI-2K）呈正相关（<0.001），与补体消耗呈负相关（<0.001）。抗Sm和抗核糖体P自身抗体与SLEDAI-2K呈正相关（分别为<0.001和=0.001），与补体消耗呈负相关（分别为<0.001和=0.001）。最后，抗Sm自身抗体与肾脏受累相关（<0.05）。
