Fernandes Sílvia P M, Vilarinho Ana Sofia, Frutuoso Amaro, Teixeira Cidália, Silva Regina Augusta A P
School of Health (ESS), Polytechnic Institute of Porto, Rua Dr. António Bernardino de Almeida, 400, 4200-072 Porto, Portugal, Rua Dr. António Bernardino de Almeida, 400, 4200-072 Porto, Portugal.
Área Técnico-Científica de Anatomia Patológica, Citológica e Tanatológica, School of Health (ESS), Rua Dr. António Bernardino de Almeida, 400, 4200-072 Porto, Portugal.
J Cytol. 2024 Apr-Jun;41(2):67-74. doi: 10.4103/joc.joc_108_23. Epub 2024 Apr 24.
kit (DiaPath S.p.A.) offers a major advantage compared to other commercially available kits available for the screening of cervical cancer, as it does not require additional equipment for sample processing. Using this methodology, collected epithelial cells are immersed in a preservative liquid before setting as a thin layer on a slide via gravity sedimentation.
To evaluate the suitability of the kit for the processing of cervical samples, detection of pre-neoplastic lesions, and nucleic preservation and extraction for HR-HPV diagnosis.
A total of 242 self-sampled cervical specimens were utilized, with 192 collected in vials and 50 collected and processed using the for comparative analysis. The samples underwent processing, Papanicolaou staining, and microscopic evaluation for morphological parameters. The extracted nucleic acids were assessed for purity and integrity, and the detection of high-risk human papillomavirus (HR-HPV) was carried out using the Alinity HR HPV system kit (Abbott Laboratórios Lda).
Both methods demonstrated effective performance, enabling the morphological assessment of the cervical epithelium. Statistical analysis indicated that yielded significantly better results in terms of cellularity. Conversely, exhibited superior performance in terms of the quantity of extracted DNA and its degree of purification. Concerning the time consumed during processing, both methods were comparable, with the methodology standing out for its cost-effectiveness, as it does not necessitate additional instruments and consumables.
The novel methodology proves effective in preserving both nucleic acids and cell morphology characteristics, two crucial features for cervical cancer screening.
与其他市售的宫颈癌筛查试剂盒相比，Kit（DiaPath S.p.A.）具有一个主要优势，即它不需要额外的设备进行样本处理。使用这种方法，收集的上皮细胞在通过重力沉降在载玻片上形成薄层之前，先浸入保存液中。
评估该试剂盒用于宫颈样本处理、癌前病变检测以及用于高危型人乳头瘤病毒（HR-HPV）诊断的核酸保存和提取的适用性。
总共使用了242份自行采集的宫颈标本，其中192份收集在小瓶中，50份使用[试剂盒名称未给出]收集和处理以进行比较分析。对样本进行处理、巴氏染色，并对形态学参数进行显微镜评估。对提取的核酸进行纯度和完整性评估，并使用Alinity HR HPV系统试剂盒（雅培实验室有限公司）进行高危型人乳头瘤病毒（HR-HPV）检测。
两种方法均表现出有效的性能，能够对宫颈上皮进行形态学评估。统计分析表明，[试剂盒名称未给出]在细胞数量方面产生的结果明显更好。相反，[另一种试剂盒名称未给出]在提取的DNA数量及其纯化程度方面表现更优。关于处理过程中消耗的时间，两种方法相当，[试剂盒名称未给出]方法因其成本效益突出，因为它不需要额外的仪器和耗材。
新的[试剂盒名称未给出]方法在保存核酸和细胞形态特征方面被证明是有效的，这是宫颈癌筛查的两个关键特征。
