School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408100, P.R. China.
College of Modern Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408100, P.R. China.
Int J Med Mushrooms. 2024;26(5):43-57. doi: 10.1615/IntJMedMushrooms.2024053182.
Wild resources of Auricularia cornea (A. polytricha) are abundant in China, and genetic diversity and genetic relationships analysis of A. cornea can provide basis for germplasm resource utilization and innovation and molecular marker-assisted breeding. In this study, 22 Auricularia strains collected were identified as A. cornea based on ITS sequence analysis, and its genetic diversity was examined by ISSR and SRAP markers. The results showed that a total of 415 bands were amplified by 11 selected ISSR primers, with an average amplification of 37.73 bands per primer, and the mean values of Ne, I, and H were 1.302, 0.368, and 0.219, respectively. A total of 450 bands were amplified by 10 SRAP primers, with an average of 45 bands per primer, and the average of Ne, I, and H were 1.263, 0.302, and 0.183, respectively. The unweighted pair-group method with arithmetic means analysis based on ISSR-SRAP marker data revealed that the genetic similarity coefficient between the tested strains was 0.73-0.97, and the strains could be divided into five groups at 0.742, which had a certain correlation with regional distribution. The results of PCOA and population structure analysis based on ISSR-SRAP data also produced similar results. These results demonstrate the genetic diversity and distinctness among wild A. cornea and provide a theoretical reference for the classification, breeding, germplasm innovation, utilization, and variety protection of A. cornea resources.
中国野生银耳（ Auricularia polytricha ）资源丰富，对银耳遗传多样性和遗传关系的分析可为种质资源的利用和创新以及分子标记辅助育种提供基础。本研究通过 ITS 序列分析，从中国 22 个银耳菌株中鉴定出银耳，并用 ISSR 和 SRAP 标记检测其遗传多样性。结果表明，11 个 ISSR 引物共扩增出 415 条带，平均每个引物扩增 37.73 条带，Ne、I 和 H 的平均值分别为 1.302、0.368 和 0.219。10 个 SRAP 引物共扩增出 450 条带，平均每个引物扩增 45 条带，Ne、I 和 H 的平均值分别为 1.263、0.302 和 0.183。基于 ISSR-SRAP 标记数据的非加权对群算术平均法分析表明，供试菌株间的遗传相似系数为 0.73-0.97，菌株在 0.742 处可分为 5 组，与区域分布有一定相关性。基于 ISSR-SRAP 数据的 PCOA 和种群结构分析结果也产生了相似的结果。这些结果表明，野生银耳具有丰富的遗传多样性和独特性，为银耳资源的分类、育种、种质创新、利用和品种保护提供了理论参考。
