Xu Liping, Lu Yunhai, You Qian, Liu Xiaolan, Grisham Michael Paul, Pan Yongbao, Que Youxiong
Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture/Fujian Agriculture and Forestry University, Fuzhou 350002, China.
USDA-ARS, Sugarcane Research Unit, Houma, LA 70360, USA.
ScientificWorldJournal. 2014 Mar 17;2014:296020. doi: 10.1155/2014/296020. eCollection 2014.
A total of 100 Sporisorium scitamineum isolates were investigated by inter simple sequence repeat (ISSR) and single primer-sequence related amplified polymorphism (SP-SRAP) markers. These isolates were clearly assorted into three distinct clusters regardless of method used: either cluster analysis or by principal component analysis (PCA) of the ISSR, SP-SRAP, or ISSR + SP-SRAP data set. The total gene diversity (H t) and gene diversity between subpopulations (H s) were estimated to be 0.34 to 0.38 and 0.22 to 0.29, respectively, by analyzing separately the ISSR and SP-SRAP data sets, and to be 0.26-0.36 by analyzing ISSR + SP-SRAP data set. The gene diversity attributable to differentiation among populations (G st) was estimated to be 0.35 and 0.22, and the gene flow (Nm) was 0.94 and 1.78, respectively, when analyzing separately ISSR and SP-SRAP data set, and was 0.27 and 1.33, respectively, when analyzing ISSR + SP-SRAP data set. Our study showed that there is considerable genetic variation in the analyzed 100 isolates, and the environmental heterogeneity has played an important role for this observed high degree of variation. The genetic differentiation of sugarcane smut fungus depends to a large extent on the heterogeneity of their habitats and is the result of long-term adaptations of pathogens to their ecological environments.
利用简单重复序列区间（ISSR）和单引物序列相关扩增多态性（SP-SRAP）标记对100株甘蔗黑粉菌分离株进行了研究。无论采用何种方法，即通过对ISSR、SP-SRAP或ISSR + SP-SRAP数据集进行聚类分析或主成分分析（PCA），这些分离株都被明显分为三个不同的簇。通过分别分析ISSR和SP-SRAP数据集，总基因多样性（H t）和亚群间基因多样性（H s）估计分别为0.34至0.38和0.22至0.29，通过分析ISSR + SP-SRAP数据集，其值为0.26 - 0.36。在分别分析ISSR和SP-SRAP数据集时，群体间分化引起的基因多样性（G st）估计分别为0.35和0.22，基因流（Nm）分别为0.94和1.78，在分析ISSR + SP-SRAP数据集时，G st分别为0.27和1.33。我们的研究表明，在所分析的100株分离株中存在相当大的遗传变异，环境异质性对观察到的这种高度变异起到了重要作用。甘蔗黑粉菌的遗传分化在很大程度上取决于其栖息地的异质性，是病原体长期适应其生态环境的结果。
