ZEB1-AS1 通过靶向 miR-320b/BMPR1A 轴在肺癌中介导骨转移。

ZEB1-AS1 mediates bone metastasis through targeting miR-320b/BMPR1A axis in lung cancer.

机构信息

Department of Cardiothoracic Vascular Surgery, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou, Hunan, China.

Department of Orthopedics, Zhuzhou Hospital Affiliated to Xiangya School of Medicine, Central South University, Zhuzhou, Hunan, China.

出版信息

Clin Respir J. 2024 May;18(5):e13770. doi: 10.1111/crj.13770.

DOI:10.1111/crj.13770

PMID:38783645

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11116843/

Abstract

OBJECTIVE

This study aimed to explore the role and regulatory mechanism of lncRNA ZEB1-AS1 in lung cancer.

METHODS

The expression of ZEB1-AS1 and miR-320b was determined by qRT-PCR. Cell viability, proliferation migration, and invasion were assessed using the CCK-8, colony-forming, and Transwell assay. EMT markers were quantified using western blot. The growth of subcutaneous tumor growth and metastatic bone tumors was evaluated in mouse model of lung cancer. Additionally, metastatic bone tumors were examined using H&E staining.

RESULTS

ZEB1-AS1 expression was upregulated, while miR-320b levels were downregulated in lung cancer. Knockdown of ZEB1-AS1 resulted in a significant suppression of cell viability, proliferation, migration, invasion, and EMT in A549 cells. Furthermore, we confirmed the targeting relationship between ZEB1-AS1 and miR-320b, as well as between miR-320b and BMPR1A. Our findings suggested that ZEB1-AS1 regulated cell viability, proliferation, migration, and invasion, as well as EMT, in lung cancer cells by targeting the miR-320b/BMPR1A axis. Moreover, our in vivo experiments confirmed that ZEB1-AS1 mediated bone metastasis through targeting miR-320b/BMPR1A axis in mice with lung cancer.

CONCLUSION

ZEB1-AS1 mediated bone metastasis through targeting miR-320b/BMPR1A axis in lung cancer.

摘要

目的

本研究旨在探讨长链非编码 RNA ZEB1-AS1 在肺癌中的作用及其调控机制。

方法

采用 qRT-PCR 检测 ZEB1-AS1 和 miR-320b 的表达。用 CCK-8、集落形成和 Transwell 实验评估细胞活力、增殖、迁移和侵袭。用 Western blot 定量 EMT 标志物。在肺癌小鼠模型中评估皮下肿瘤生长和转移性骨肿瘤的生长。此外，用 H&E 染色检查转移性骨肿瘤。

结果

肺癌中 ZEB1-AS1 表达上调，miR-320b 水平下调。A549 细胞中 ZEB1-AS1 敲低导致细胞活力、增殖、迁移、侵袭和 EMT 显著抑制。此外，我们证实了 ZEB1-AS1 与 miR-320b 之间以及 miR-320b 与 BMPR1A 之间的靶向关系。我们的研究结果表明，ZEB1-AS1 通过靶向 miR-320b/BMPR1A 轴调控肺癌细胞的活力、增殖、迁移和侵袭以及 EMT。此外，我们的体内实验证实，ZEB1-AS1 通过靶向 miR-320b/BMPR1A 轴在肺癌小鼠中介导骨转移。