Department of Orthodontics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Department of Medical Imaging, Qingdao Hiser Hospital Affiliated of Qingdao University (Qingdao Traditional Chinese Medicine Hospital) Qingdao, Shandong 266003, P.R. China.
Mol Med Rep. 2024 Jul;30(1). doi: 10.3892/mmr.2024.13249. Epub 2024 May 24.
Periodontal disease is a common infectious disease that can lead to the loss of teeth. Hower how to effectively suppress the inflammation with medication is unclear. The aim of the present study was to investigate the anti‑inflammatory effect of Oroxylin A in periodontitis and its potential role through heme oxygenase‑1 (HO‑1). Primary rat gingival fibroblasts (RGFs) were cultured using the tissue block method and identified by immunofluorescence. Following lipopolysaccharide (LPS) stimulation of RGFs, Oroxylin A was administered at 50, 100, 200 or 400 µg/ml. Reverse transcription‑quantitative PCR was used to assess mRNA expression of cyclooxygenase (COX)‑2, TNF‑α, RANKL and osteoprotegerin (OPG). Western blotting was used to detect protein expression levels of COX ‑2, TNF‑α, RANKL and OPG. Following HO‑1 knockdown, the same treatment was performed. The expression of COX‑2 in rat gingival tissue was observed by immunohistochemistry. One‑way analysis of variance and Student's t test were used for statistical analysis. Oroxylin A downregulated mRNA expression of COX‑2, TNF‑α, RANKL and OPG in LPS‑induced RGFs. With increase of Oroxylin A dose, the expression of HO‑1 was gradually upregulated. When HO‑1 was knocked down, Oroxylin A did not downregulate the expression of COX‑2, TNF‑α, RANKL and OPG in LPS‑induced RGFs. Immunohistochemical results showed that expression of COX‑2 was downregulated by Oroxylin A, and the expression of TNF‑α, RANKL and OPG were also downregulated. Oroxylin A decreased expression of inflammatory cytokines in LPS‑induced RGFs and had a good inhibitory effect on periodontitis in rats.
牙周病是一种常见的传染病，可导致牙齿丧失。然而，如何通过药物有效抑制炎症尚不清楚。本研究旨在探讨白杨素 A 对牙周炎的抗炎作用及其通过血红素加氧酶-1 (HO-1) 发挥作用的潜在机制。采用组织块法培养大鼠牙龈成纤维细胞（RGFs），并通过免疫荧光法进行鉴定。用脂多糖（LPS）刺激 RGFs 后，用 50、100、200 或 400μg/ml 的白杨素 A 进行处理。采用反转录-定量 PCR 检测环氧化酶（COX）-2、TNF-α、RANKL 和骨保护素（OPG）的 mRNA 表达。采用 Western blot 检测 COX-2、TNF-α、RANKL 和 OPG 的蛋白表达水平。在 HO-1 敲低后，进行相同的处理。采用免疫组织化学法观察 COX-2 在大鼠牙龈组织中的表达。采用单因素方差分析和学生 t 检验进行统计学分析。白杨素 A 下调 LPS 诱导的 RGFs 中 COX-2、TNF-α、RANKL 和 OPG 的 mRNA 表达。随着白杨素 A 剂量的增加，HO-1 的表达逐渐上调。当 HO-1 被敲低时，白杨素 A 不能下调 LPS 诱导的 RGFs 中 COX-2、TNF-α、RANKL 和 OPG 的表达。免疫组织化学结果表明，白杨素 A 下调 COX-2 的表达，同时下调 TNF-α、RANKL 和 OPG 的表达。白杨素 A 降低 LPS 诱导的 RGFs 中炎症细胞因子的表达，对大鼠牙周炎具有良好的抑制作用。
