免疫组织化学作为意大利撒丁岛患者囊性棘球蚴病诊断的可靠工具——一项验证性研究
Immunohistochemistry as a Reliable Tool for the Diagnosis of Cystic Echinococcosis in Patients from Sardinia, Italy-A Confirmatory Study.
作者信息
Santucciu Cinzia, Peruzzu Angela, Fara Antonella Maria, Cossu Antonio, Kronenberg Philipp A, Deplazes Peter, Masala Giovanna
机构信息
WOAH and National Reference Laboratory for Echinococcosis, Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, Italy.
Department of Biomedical Sciences, Institute of Pathology, University of Sassari, 07100 Sassari, Italy.
出版信息
Diseases. 2024 Apr 26;12(5):84. doi: 10.3390/diseases12050084.
Cystic Echinococcosis (CE) is a zoonotic disease caused by the larval stage of the tapeworm . This study aims to investigate the use of two monoclonal antibodies (mAbEmG3 and mAbEm2G11) by immunohistochemistry (IHC) to confirm the diagnosis of CE in human patients, in particular in those cases in which other techniques fail to provide a correct or conclusive diagnosis. For this purpose, a survey on 13 patients was performed. These subjects were referred to Sardinian hospitals (Italy) from 2017 to 2022 and were suspected to be affected by CE. Our findings from these 13 patients showed the detection of by IHC in 12 of 13 echinococcal cysts, as one sample was of a non-parasitological origin. The results confirmed that IHC, by means of the mAbEmG3 and mAbEm2G11, is a reliable diagnostic tool that showed a very high performances when tested on strain of from Sardinia.
囊型包虫病（CE）是一种由绦虫幼虫阶段引起的人畜共患疾病。本研究旨在探讨通过免疫组织化学（IHC）使用两种单克隆抗体（mAbEmG3和mAbEm2G11）来确诊人类患者的囊型包虫病，特别是在其他技术无法提供正确或确定性诊断的情况下。为此，对13名患者进行了一项调查。这些受试者在2017年至2022年期间被转诊至意大利撒丁岛的医院，疑似感染囊型包虫病。我们对这13名患者的研究结果显示，在13个棘球蚴囊肿中的12个中通过免疫组织化学检测到了[此处原文缺失相关内容]，因为有一个样本来源非寄生虫学。结果证实，通过mAbEmG3和mAbEm2G11进行的免疫组织化学是一种可靠的诊断工具，在对来自撒丁岛的[此处原文缺失相关内容]菌株进行测试时表现出非常高的性能。
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