源自狭义细粒棘球绦虫的天然包虫囊肿液（HCF）、冻干HCF、抗原B（AgB）和冻干AgB（LAgB）用于人类肝囊性棘球蚴病活动期、过渡期和非活动期血清诊断的比较

Comparison of Native Hydatid Cyst Fluid (HCF), Lyophilized HCF, Antigen B (AgB) and Lyophilized AgB (LAgB) Originated from Sensu Stricto for Sero-Diagnosis of Active, Transitional and Inactive Human Liver Cystic Echinococcosis.

作者信息

Sadjjadi Fatemeh Sadat, Mohammadzadeh Tahereh, Jafari Seyed Hamed, Sharifi Yosef, Deilami Hamed Nikoupour, Hafezi Fatemeh, Sadjjadi Seyed Mahmoud

机构信息

School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baghiyatallah University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Public Health. 2023 Aug;52(8):1764-1772. doi: 10.18502/ijph.v52i8.13416.

DOI:10.18502/ijph.v52i8.13416

PMID:37744545

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10512133/

Abstract

BACKGROUND

Cystic echinococcosis (CE) is an important zoonotic parasitic disease caused by the larval stage or metacestode of the tapeworm sensu lato. Due to treatment protocols for different liver cysts, diagnosis of cyst stages is very important. Different antigens have been used for CE diagnosis. However, each one is more sensitive and effective for the diagnosis of specific CE stages is not known well. We aimed to compare Native Hydatid Cyst Fluid (HCF), Lyophilized Hydatid Cyst Fluid (LHCF), antigen B (AgB) and Lyophilized antigen B (LAgB) originated from sensu stricto (G1-G3) genotype, for sero- diagnosis of active, transitional and inactive human liver CE using ELISA technique.

METHODS

The HCF was collected aseptically from liver CE cysts of sheep slaughtered from 2018 to 2019 in Shiraz slaughterhouse, Southern, Iran. The cysts were characterized by PCR and sequencing for genotype specification. Four types of antigens were used: HCF, LHCF, AgB and LAgB originated from sensu stricto (G1-G3) genotype. Thirty-three serum samples from active, transitional, and inactive human cysts were collected. Overall, 48 samples from other parasitic diseases and 60 samples from healthy subjects as negative controls were checked using four antigens by ELISA method.

RESULTS

The best diagnostic sensitivity with 96.97% was observed by anti-LHCF IgG ELISA test. The best specificity with 95.37% was observed in ELISA test using LAgB.

CONCLUSION

Simultaneous test of sera with anti-LHCF IgG ELISA and anti-LAgB IgG ELISA would be the best in the diagnosis of human liver cystic echinococcosis.

摘要

背景

囊型包虫病（CE）是由绦虫幼虫期或中绦期引起的一种重要的人畜共患寄生虫病。由于不同肝囊肿的治疗方案不同，囊肿分期的诊断非常重要。不同抗原已被用于CE的诊断。然而，每种抗原对特定CE分期诊断的敏感性和有效性尚不十分清楚。我们旨在比较源自狭义（G1 - G3）基因型的天然包虫囊肿液（HCF）、冻干包虫囊肿液（LHCF）、抗原B（AgB）和冻干抗原B（LAgB），采用酶联免疫吸附测定（ELISA）技术对人类活动性、过渡性和非活动性肝CE进行血清学诊断。

方法

2018年至2019年在伊朗南部设拉子屠宰场屠宰的绵羊肝脏CE囊肿中无菌采集HCF。通过聚合酶链反应（PCR）和测序对囊肿进行基因型鉴定。使用了四种类型的抗原：源自狭义（G1 - G3）基因型的HCF、LHCF、AgB和LAgB。收集了33份来自人类活动性、过渡性和非活动性囊肿的血清样本。总体而言，使用ELISA方法用四种抗原检测了48份来自其他寄生虫病的样本和60份来自健康受试者的样本作为阴性对照。