Fimbres-García Jorge O, Flores-Sauceda Marcela, Othón-Díaz Elsa Daniela, García-Galaz Alfonso, Tapia-Rodriguez Melvin R, Silva-Espinoza Brenda A, Alvarez-Armenta Andres, Ayala-Zavala J Fernando
Centro de Investigación en Alimentación y Desarrollo, A.C, Carretera Gustavo Enrique Astiazarán Rosas 46, Hermosillo 83304, Sonora, Mexico.
Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, Col. Centro, Ciudad Obregón 85000, Sonora, Mexico.
Antibiotics (Basel). 2024 May 14;13(5):444. doi: 10.3390/antibiotics13050444.
This research focuses on assessing the synergistic effects of Mexican oregano ( essential oil or carvacrol when combined with the antibiotic imipenem, aiming to reduce the pathogenic viability and virulence of and . The study highlighted the synergistic effect of combining essential oil or carvacrol with imipenem, significantly reducing the required doses for inhibiting bacterial growth. The combination treatments drastically lowered the necessary imipenem doses, highlighting a potent enhancement in efficacy against and . For example, the minimum inhibitory concentrations (MIC) for the essential oil/imipenem combinations were notably low, at 0.03/0.000023 mg/mL for and 0.0073/0.000023 mg/mL for . Similarly, the combinations significantly inhibited biofilm formation at lower concentrations than when the components were used individually, demonstrating the strategic advantage of this approach in combating antibiotic resistance. For OXA-51, imipenem showed a relatively stable interaction during 30 ns of dynamic simulation of their interaction, indicating changes (<2 nm) in ligand positioning during this period. Carvacrol exhibited similar fluctuations to imipenem, suggesting its potential inhibition efficacy, while thymol showed significant variability, particularly at >10 ns, suggesting potential instability. With IMP-1, imipenem also displayed very stable interactions during 38 ns and demonstrated notable movement and positioning changes within the active site, indicating a more dynamic interaction. In contrast, carvacrol and thymol maintained their position within the active site only ~20 and ~15 ns, respectively. These results highlight the effectiveness of combining essential oil and carvacrol with imipenem in tackling the difficult-to-treat pathogens and .
本研究着重评估墨西哥牛至（精油或香芹酚）与抗生素亚胺培南联合使用时的协同效应，旨在降低[具体病原体1]和[具体病原体2]的致病活力和毒力。该研究突出了墨西哥牛至精油或香芹酚与亚胺培南联合使用的协同效应，显著降低了抑制细菌生长所需的剂量。联合治疗大幅降低了所需的亚胺培南剂量，凸显了对[具体病原体1]和[具体病原体2]的疗效显著增强。例如，精油/亚胺培南组合的最低抑菌浓度（MIC）显著较低，[具体病原体1]为0.03/0.000023毫克/毫升，[具体病原体2]为0.0073/0.000023毫克/毫升。同样，与单独使用各成分相比，联合使用在更低浓度下就能显著抑制生物膜形成，证明了这种方法在对抗抗生素耐药性方面的战略优势。对于OXA - 51，在其相互作用的30纳秒动态模拟过程中，亚胺培南表现出相对稳定的相互作用，表明在此期间配体定位变化（<2纳米）。香芹酚与亚胺培南表现出相似的波动，表明其潜在的抑制效果，而百里香酚表现出显著的变异性，特别是在>10纳秒时，表明潜在的不稳定性。对于IMP - 1，亚胺培南在38纳秒内也表现出非常稳定的相互作用，并在活性位点内显示出显著的移动和定位变化，表明相互作用更具动态性。相比之下，香芹酚和百里香酚分别仅在活性位点内保持其位置约20纳秒和15纳秒。这些结果突出了墨西哥牛至精油和香芹酚与亚胺培南联合使用在应对难治疗病原体[具体病原体1]和[具体病原体2]方面的有效性。
