Department of Microbiology, Aggeu Magalhães Institute - Oswaldo Cruz Foundation, Recife, PE, Brazil.
Federal University of Pernambuco, CEP 50670-901, Recife, PE, Brazil.
Chem Biodivers. 2024 Oct;21(10):e202400537. doi: 10.1002/cbdv.202400537. Epub 2024 Sep 11.
To assess the antibacterial effectiveness of Lippia macrophylla essential oil (LMEO) against multidrug-resistant Acinetobacter baumannii isolates, both as a standalone treatment and in combination with conventional antibiotics. LMEO demonstrated a significant inhibitory effect on the growth of A. baumannii, with a minimum inhibitory concentration (MIC) below 500 μg/mL. Notably, LMEO was capable of reversing the antibiotic resistance of clinical isolates or reducing their MIC values when used in combination with antibiotics, showing synergistic (FICI≤0.5) or additive effects. The combination of LMEO and imipenem was particularly effective, displaying synergistic interactions for most isolates. Ultrastructural analyses supported these findings, revealing that the combination of LMEO+ceftazidime compromised the membrane integrity of the Acb35 isolate, leading to cytoplasmic leakage and increased formation of Outer Membrane Vesicles (OMVs). Taken together our results point for the use of LMEO alone or in combination as an antibacterial agent against A. baumannii. These findings offer promising avenues for utilizing LMEO as a novel antibacterial strategy against drug-resistant infections in healthcare settings, underscoring the potential of essential oils in enhancing antibiotic efficacy.
为评估大花九里香精油（LMEO）作为单一药物及与传统抗生素联合使用对多重耐药鲍曼不动杆菌的抗菌效果。LMEO 对鲍曼不动杆菌的生长表现出显著的抑制作用，最低抑菌浓度（MIC）低于 500μg/ml。值得注意的是，LMEO 能够逆转临床分离株的抗生素耐药性，或者与抗生素联合使用时降低其 MIC 值，显示出协同（FICI≤0.5）或相加作用。LMEO 与亚胺培南的组合尤其有效，对大多数分离株显示出协同相互作用。超微结构分析支持了这些发现，表明 LMEO+头孢他啶的组合破坏了 Acb35 分离株的膜完整性，导致细胞质渗漏和外膜囊泡（OMVs）的形成增加。综上所述，我们的研究结果表明，LMEO 可单独或联合用作抗鲍曼不动杆菌的抗菌剂。这些发现为在医疗环境中利用 LMEO 作为一种针对耐药感染的新型抗菌策略提供了有前景的途径，强调了精油在增强抗生素疗效方面的潜力。
