评估FlukeCatcher检测泌尿生殖系统血吸虫病的性能。
Evaluating the Performance of FlukeCatcher at Detecting Urogenital Schistosomiasis.
作者信息
Fok Louis, Erko Berhanu, Brett-Major David, Animut Abebe, Broadhurst M Jana, Dai Daisy, Linville John, Levecke Bruno, Negash Yohannes, Degarege Abraham
机构信息
Department of Epidemiology, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia.
出版信息
Diagnostics (Basel). 2024 May 17;14(10):1037. doi: 10.3390/diagnostics14101037.
Urine filtration microscopy (UFM) lacks sensitivity in detecting low-intensity infections. In pursuit of a superior alternative, this study evaluated the performance of FlukeCatcher microscopy (FCM) at detecting eggs in human urine samples. Urine samples were collected from 572 school-age children in Afar, Ethiopia in July 2023 and examined using UFM and FCM approaches. Using the combined UFM and FCM results as a reference, the sensitivity, negative predictive value, and agreement levels for the two testing methods in detecting eggs in urine samples were calculated. The sensitivity and negative predictive value of detecting eggs in urine samples for FCM was 84% and 97%, respectively, compared to 65% and 93% for UFM. The FCM test results had an agreement of 61% with the UFM results, compared to 90% with the combined results of FCM and UFM. However, the average egg count estimates were lower when using FCM (6.6 eggs per 10 mL) compared to UFM (14.7 eggs per 10 mL) ( < 0.0001). Incorporating FCM into specimen processing could improve the diagnosis of infection but may underperform in characterizing the intensity of infection.
尿液过滤显微镜检查（UFM）在检测低强度感染方面缺乏敏感性。为了寻求更好的替代方法，本研究评估了吸虫捕获显微镜检查（FCM）在检测人类尿液样本中虫卵方面的性能。2023年7月，从埃塞俄比亚阿法尔地区的572名学龄儿童中收集尿液样本，并使用UFM和FCM方法进行检查。以UFM和FCM的联合结果为参考，计算了两种检测方法在检测尿液样本中虫卵时的敏感性、阴性预测值和一致性水平。FCM检测尿液样本中虫卵的敏感性和阴性预测值分别为84%和97%，而UFM分别为65%和93%。FCM检测结果与UFM结果的一致性为61%，与FCM和UFM的联合结果的一致性为90%。然而，与UFM（每10 mL 14.7个虫卵）相比，使用FCM时平均虫卵计数估计值较低（每10 mL 6.6个虫卵）（<0.0001）。将FCM纳入样本处理可能会改善感染的诊断，但在确定感染强度方面可能表现不佳。
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