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基于 GC-MS/FID 的甲基化方法分析红海藻类未分级多糖的糖苷键连接方式。

Methylation-GC-MS/FID-Based Glycosidic Linkage Analysis of Unfractionated Polysaccharides in Red Seaweeds.

机构信息

Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Avenue South, Lethbridge, AB T1J 4B1, Canada.

出版信息

Mar Drugs. 2024 Apr 24;22(5):192. doi: 10.3390/md22050192.

DOI:10.3390/md22050192
PMID:38786583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11122361/
Abstract

Glycosidic linkage analysis was conducted on the unfractionated polysaccharides in alcohol-insoluble residues (AIRs) prepared from six red seaweeds ( sp., sp., , sp., , and ) using GC-MS/FID analysis of partially methylated alditol acetates (PMAAs). The cell walls of primarily contained mixed-linkage xylans and small amounts of sulfated galactans and cellulose. In contrast, the unfractionated polysaccharides of the other five species were rich in galactans displaying diverse 3,6-anhydro-galactose and galactose linkages with varied sulfation patterns. Different levels of cellulose were also observed. This glycosidic linkage method offers advantages for cellulose analysis over traditional monosaccharide analysis that is known for underrepresenting glucose in crystalline cellulose. Relative linkage compositions calculated from GC-MS and GC-FID measurements showed that anhydro sugar linkages generated more responses in the latter detection method. This improved linkage workflow presents a useful tool for studying polysaccharide structural variations across red seaweed species. Furthermore, for the first time, relative linkage compositions from GC-MS and GC-FID measurements, along with normalized FID and total ion current (TIC) chromatograms without peak assignments, were analyzed using principal component analysis (PCA) as a proof-of-concept demonstration of the technique's potential to differentiate various red seaweed species.

摘要

糖苷键分析是在六种红海藻（ sp.、 sp.、 、 、 、 ）的醇不溶残渣（AIRs）中未分级的多糖上进行的，使用 GC-MS/FID 分析部分甲基化糖醇乙酸酯（PMAAs）。 主要由混合连接木聚糖和少量硫酸化半乳糖和纤维素组成。相比之下，其他五个种的未分级多糖富含半乳糖，显示出具有不同硫酸化模式的各种 3,6-脱水半乳糖和半乳糖键。还观察到不同水平的纤维素。与传统的单糖分析相比，这种糖苷键方法在纤维素分析中具有优势，因为传统的单糖分析已知会低估结晶纤维素中的葡萄糖。从 GC-MS 和 GC-FID 测量计算的相对键合组成表明，在后一种检测方法中，脱水糖键产生了更多的响应。这种改进的键合工作流程为研究红海藻种间多糖结构变化提供了有用的工具。此外，首次使用 GC-MS 和 GC-FID 测量的相对键合组成、以及没有峰分配的归一化 FID 和总离子流（TIC）色谱图，使用主成分分析（PCA）进行分析，作为该技术区分各种红海藻种潜力的概念验证演示。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffc1/11122361/09e4339027a9/marinedrugs-22-00192-g012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffc1/11122361/09e4339027a9/marinedrugs-22-00192-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffc1/11122361/f6ff677b5bbe/marinedrugs-22-00192-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffc1/11122361/b65310849bbb/marinedrugs-22-00192-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffc1/11122361/58dbceba97d2/marinedrugs-22-00192-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffc1/11122361/7e32b4623615/marinedrugs-22-00192-g003.jpg
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