Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Avenue South, Lethbridge, AB T1J 4B1, Canada.
Spoitz Enterprises Inc., 215-1610 Pandora Street, Vancouver, BC V5L 1L6, Canada.
Mar Drugs. 2024 Oct 9;22(10):464. doi: 10.3390/md22100464.
This study introduces a novel approach to analyze glycosidic linkages in unfractionated polysaccharides from alcohol-insoluble residues (AIRs) of five brown seaweed species. GC-MS analysis of partially methylated alditol acetates (PMAAs) enables monitoring and comparison of structural variations across different species, harvest years, and tissues with and without blanching treatments. The method detects a wide array of fucose linkages, highlighting the structural diversity in glycosidic linkages and sulfation position in fucose-containing sulfated polysaccharides. Additionally, this technique enhances cellulose quantitation, overcoming the limitations of traditional monosaccharide composition analysis that typically underestimates cellulose abundance due to incomplete hydrolysis of crystalline cellulose. The introduction of a weak methanolysis-sodium borodeuteride reduction pretreatment allows for the detection and quantitation of uronic acid linkages in alginates.
本研究介绍了一种分析五种褐藻属种的醇不溶残渣（AIRs）中未分级多糖的糖苷键的新方法。气相色谱-质谱联用分析部分甲基化糖醇乙酸酯（PMAAs）可用于监测和比较不同种、不同收获年份以及经和未经烫漂处理的组织之间的结构变化。该方法可检测到广泛的岩藻糖键，突出了含岩藻糖的硫酸化多糖中糖苷键和硫酸化位置的结构多样性。此外，该技术还可增强纤维素的定量分析，克服了传统单糖组成分析的局限性，后者由于结晶纤维素不完全水解，通常会低估纤维素的丰度。引入弱甲醇解-硼氢化钠还原预处理可检测和定量褐藻胶中的糖醛酸键。
