Li Xiaoyu, Zhang Shengnan, Guo Ruixue, Xiao Xuejing, Liu Boying, Mahmoud Rehab Khaled, Abukhadra Mostafa R, Qu Ruijuan, Wang Zunyao
State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
Faculty of Science, Beni Suef University, Beni Suef 62521, Egypt.
Toxics. 2024 May 13;12(5):361. doi: 10.3390/toxics12050361.
Soil contamination of polycyclic aromatic hydrocarbons (PAHs), especially caused by the mixture of two or more PAHs, raised great environmental concerns. However, research on the migration and transformation processes of PAHs in soils and their interactions with native communities is limited. In this work, soil samples from uncontaminated sites around the industrial parks in Handan, Hengshui, and Shanghai were artificially supplemented with three concentrations of anthracene (Ant), 9-chloroanthracene (9-ClAnt), benzopyrene (BaP), and chrysene (Chr). Ryegrass was planted to investigate the degradation of PAHs and its interaction with native soil organisms in the constructed ryegrass-microbe-soil microcosmic system. The bacterial and fungal communities in soil were affected by PAHs; their species diversity and relative abundance changed after exposure to different concentrations of PAHs, among which , , , and bacteria were correlated to the degradation of PAHs. On the 56th day, the contents of BaP, Chr, and Ant decreased with the degradation process, while the degradation of 9-ClAnt was limited. Nineteen intermediates, including hydroxylation and carboxylated compounds, were identified. The present research would help clarify the potential interactions between PAHs and native organisms in contaminated sites, providing fundamental information for evaluating the transformation risks of PAHs in the natural environment.
多环芳烃（PAHs）对土壤的污染，尤其是由两种或更多种多环芳烃混合造成的污染，引起了极大的环境关注。然而，关于多环芳烃在土壤中的迁移和转化过程及其与本地群落相互作用的研究有限。在本研究中，对邯郸、衡水和上海工业园区周边未受污染地点的土壤样本人工添加了三种浓度的蒽（Ant）、9-氯蒽（9-ClAnt）、苯并芘（BaP）和屈（Chr）。种植黑麦草以研究在构建的黑麦草-微生物-土壤微观系统中多环芳烃的降解及其与本地土壤生物的相互作用。土壤中的细菌和真菌群落受到多环芳烃的影响；在接触不同浓度的多环芳烃后，它们的物种多样性和相对丰度发生了变化，其中， 、 、 和 细菌与多环芳烃的降解相关。在第56天，BaP、Chr和Ant的含量随着降解过程而降低，而9-ClAnt的降解有限。鉴定出了19种中间体，包括羟基化和羧基化化合物。本研究将有助于阐明污染场地中多环芳烃与本地生物之间的潜在相互作用，为评估多环芳烃在自然环境中的转化风险提供基础信息。
