MicroRNA-186 抑制膀胱癌中的细胞增殖和转移。

MicroRNA-186 suppresses cell proliferation and metastasis in bladder cancer.

机构信息

Department of Urology, Qingdao Chengyang People's hospital, Qing dao, Shan dong Province, P.R.China.

出版信息

Afr Health Sci. 2022 Dec;22(4):56-63. doi: 10.4314/ahs.v22i4.8.

DOI:10.4314/ahs.v22i4.8

PMID:37092087

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10117455/

Abstract

PURPOSE

Bladder cancer (BCa) is a common malignancy in the urinary system. This study aims to explore the role of miR-186 in BCa tumorigenesis.

METHODS

The expression of miR-186 and ADAMTS12 in clinical BCa tissues and cell lines was detected. BCa cell lines T24, 5637 and EJ were used to transfect miR-186 mimics or inhibitors. Luciferase reporter gene detection confirmed the correlation between miR-186 and ADAMTS12. MTT method and flow cytometry were used to detect cell viability and apoptosis. Cell migration and invasion ability was detected by transwell assay. The protein level of ADAMTS12, β-catenin, GSK-3β and p-GSK-3β was determined using western blot analysis.

RESULTS

MiR-186 was negatively correlated with the expression of ADAMTS12 in BCa tissues. Further research confirmed that ADAMTS12 is the direct target of miR-186. In addition, overexpression of miR-186 down-regulated the expression of ADAMTS12, inhibiting cell viability and apoptosis, while knockout of miR-186 led to the opposite result. miR-186 also inhibits the phosphorylation of GSK-3 β and β-catenin without changing the total GSK-3β level. Our study shows that miR-186 has a negative regulatory effect on the expression of ADAMTS12 in clinical specimens and in vitro. miR-186 can inhibit the proliferation and invasion of BCa cells.

CONCLUSIONS

miR-186 has the potential to be used as a biomarker in the early detection of BCa.

摘要

目的

膀胱癌（BCa）是泌尿系统常见的恶性肿瘤。本研究旨在探讨 miR-186 在 BCa 肿瘤发生中的作用。

方法

检测临床 BCa 组织和细胞系中 miR-186 和 ADAMTS12 的表达。使用 T24、5637 和 EJ 膀胱癌细胞系转染 miR-186 模拟物或抑制剂。荧光素酶报告基因检测证实 miR-186 与 ADAMTS12 之间的相关性。MTT 法和流式细胞术检测细胞活力和凋亡。Transwell 检测细胞迁移和侵袭能力。Western blot 分析检测 ADAMTS12、β-catenin、GSK-3β 和 p-GSK-3β 的蛋白水平。

结果

miR-186 与 BCa 组织中 ADAMTS12 的表达呈负相关。进一步研究证实 ADAMTS12 是 miR-186 的直接靶标。此外，miR-186 的过表达下调 ADAMTS12 的表达，抑制细胞活力和凋亡，而 miR-186 的敲除则导致相反的结果。miR-186 还抑制 GSK-3β 和 β-catenin 的磷酸化，而不改变总 GSK-3β 水平。我们的研究表明，miR-186 对临床标本和体外 ADAMTS12 的表达具有负调控作用。miR-186 可抑制 BCa 细胞的增殖和侵袭。