Sibula Madeline Siyazisiwe, Malatji Mokgadi Pulane, Nyahunda Cosmas, Mukaratirwa Samson
School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Westville Campus, Durban 4001, South Africa.
Department of Applied Biology and Biochemistry, Faculty of Applied Science, National University of Science and Technology, Bulawayo P.O. Box AC939, Zimbabwe.
Vet Sci. 2024 May 13;11(5):211. doi: 10.3390/vetsci11050211.
This study aimed at determining the identity of freshwater snails collected from selected water habitats frequented by wildlife as source of drinking water in the Matebeleland region of Zimbabwe and further screening the identified snails for natural infections with amphistomes using PCR. A total of 487 freshwater snails were collected from six areas in the Matebeleland region of Zimbabwe for identification and screening of amphistome infection. Eight freshwater snail species were morphologically identified and , and were confirmed using the COI gene. and were the most abundant species at 33.9% (165/487) and 31.2% (155/487), respectively. DNA of amphistome was detected in 11.9% (58/487) of the collected snails. The highest infection rate was detected in (44.4%). West Nicholson recorded the highest infection rate (33.9%), and infection was not detected in , , and spp. Amphistome DNA from was successfully sequenced and identified as . An additional band was detected in , , and , which showed a 96.42% similarity to sp. sequence in the GenBank.
本研究旨在确定从津巴布韦马塔贝莱兰地区野生动物常去的作为饮用水源的特定水生生境中采集的淡水螺的种类，并进一步使用聚合酶链反应（PCR）对已鉴定的螺进行自然感染双口吸虫的筛查。从津巴布韦马塔贝莱兰地区的六个区域共采集了487只淡水螺，用于双口吸虫感染的鉴定和筛查。通过形态学鉴定出了八种淡水螺，并使用细胞色素氧化酶亚基I（COI）基因进行了确认。其中两种螺最为常见，分别占33.9%（165/487）和31.2%（155/487）。在所采集的螺中，11.9%（58/487）检测到双口吸虫的DNA。感染率最高的是[具体螺名1]（44.4%）。西尼科尔森的感染率最高（33.9%），而在[具体螺名2]、[具体螺名3]和[具体螺名4]中未检测到感染。来自[具体螺名5]的双口吸虫DNA成功测序并鉴定为[具体种类]。在[具体螺名6]、[具体螺名7]和[具体螺名8]中检测到一条额外的条带，其与GenBank中[某种双口吸虫]序列的相似度为96.42%。
