Mattes M J, Sharrow S O, Herberman R B, Holden H T
J Immunol. 1979 Dec;123(6):2851-60.
The expression of the Thy-1 antigen on mouse spleen cells responsible for NK activity and ADCC was investigated by using a monoclonal IgM anti-Thy-1.2 antibody. Both C-mediated cytotoxicity and the fluorescence-activated cell sorter were used to fractionate cells. The effector cells were found to be heterogeneous in their expression of Thy-1. Effector cells from nude BALB/c mice were predominantly Thy-1 positive; some of the NK cells in CBA spleens appeared to be Thy-1 positive, but at least one-third of the lytic activity was due to Thy-1 negative cells. The effects of treatments on NK cytotoxicity and ADCC were very similar, supporting the hypothesis that the same cells mediate both activities.
利用单克隆IgM抗Thy-1.2抗体研究了负责自然杀伤（NK）活性和抗体依赖的细胞介导的细胞毒性（ADCC）的小鼠脾细胞上Thy-1抗原的表达。补体（C）介导的细胞毒性和荧光激活细胞分选仪均用于细胞分级分离。发现效应细胞在Thy-1表达上具有异质性。裸BALB/c小鼠的效应细胞主要为Thy-1阳性；CBA脾中的一些NK细胞似乎是Thy-1阳性，但至少三分之一的裂解活性归因于Thy-1阴性细胞。处理对NK细胞毒性和ADCC的影响非常相似，支持了相同细胞介导这两种活性的假说。
