Department of Mechatronics, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal 576104, India.
Department of Instrumentation and Control, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal 576104, India.
Nanotechnology. 2024 Jun 10;35(34). doi: 10.1088/1361-6528/ad501b.
Rampant pathogenesis induced by communicable microbes has necessitated development of technologies for rapid and sustained disinfection of surfaces. Copper nanoparticles (CuNPs) have been widely reported for their antimicrobial properties. However, nanostructured copper is prone to oxidative dissolution in the oil phase limiting its sustained use on surfaces and coatings. The current study reports a systematic investigation of a simple synthesis protocol using fatty acid stabilizers (particularly essential oils) for synthesis of copper nanoparticles in the oil phase. Of the various formulations synthesized, rosemary oil stabilized copper nanoparticles (RMO CuNPs) were noted to have the best inactivation kinetics and were also most stable. Upon morphological characterization by TEM and EELS, these were found to be monodispersed (5-8 nm) with copper coexisting in all three oxidation states on the surface of the nanoparticles. The nanoparticles were drop cast on woven fabric of around 500 threads per inch and exposed to gram positive bacteria (), gram negative bacteria (and), enveloped RNA virus (phi6), non-enveloped RNA virus (MS2) and non-enveloped DNA virus (T4) to encompass the commonly encountered groups of pathogens. It was possible to completely disinfect 10copies of all microorganisms within 40 min of exposure. Further, this formulation was incorporated with polyurethane as thinners and used to coat non-woven fabrics. These also exhibited antimicrobial properties. Sustained disinfection with less than 9% cumulative copper loss for upto 14 washes with soap water was observed while the antioxidant activity was also preserved. Based on the studies conducted, RMO CuNP in oil phase was found to have excellent potential of integration on surface coatings, paints and polymers for rapid and sustained disinfection of microbes on surfaces.
传染性微生物引起的猖獗发病，促使人们开发出快速、持续消毒表面的技术。纳米铜颗粒（CuNPs）因其抗菌性能而被广泛报道。然而，纳米结构的铜在油相中容易发生氧化溶解，限制了其在表面和涂层上的持续使用。本研究报告了一种使用脂肪酸稳定剂（特别是精油）在油相中合成铜纳米颗粒的系统研究。在所合成的各种配方中，迷迭香油稳定的铜纳米颗粒（RMO CuNPs）被发现具有最佳的失活动力学，并且也是最稳定的。通过 TEM 和 EELS 的形态特征分析，发现这些纳米颗粒呈单分散性（5-8nm），铜以三种氧化态共存于纳米颗粒的表面。将纳米颗粒滴铸在每英寸约 500 根线的机织织物上，并将其暴露于革兰氏阳性菌（）、革兰氏阴性菌（和）、包膜 RNA 病毒（phi6）、非包膜 RNA 病毒（MS2）和非包膜 DNA 病毒（T4）中，以涵盖常见的病原体。在暴露 40 分钟内，就可以完全消灭所有微生物的 10 个拷贝。此外，该配方与聚氨酯一起作为稀释剂，用于涂覆非织造织物。这些也表现出抗菌性能。在 14 次肥皂水洗中，铜的累积损失小于 9%，持续消毒，同时保持抗氧化活性。根据进行的研究，发现油相中的 RMO CuNP 具有在表面涂层、油漆和聚合物上进行集成的巨大潜力，可实现表面微生物的快速和持续消毒。
