Kopitar M, Rozman B, Babnik J, Turk V, Mullins D E, Wun T C
Thromb Haemost. 1985 Dec 17;54(4):750-5.
A plasminogen activator inhibitor (PA-I) which inhibits primarily plasminogen activator of the urokinase type (u-PA) was isolated from the cytosol of human peripheral leukocytes. The inhibitor was isolated using ion exchange chromatography, gel filtration and FPLC. This inhibitor has an apparent molecular weight of 45 kDa, determined by SDS-PAGE, and a pI of 5.5-5.7. The inhibitor is a fast reacting inhibitor, is thermally unstable and is inactivated outside the pH range 7-9. Treatment of cytosol to pH 9 for 30 min at 37 degrees C resulted in a large increase in inhibitory activity. Antibodies against human placental UK-I completely quenched the inhibitory activity of human leucocyte UK-I.
从人外周血白细胞胞质溶胶中分离出一种主要抑制尿激酶型纤溶酶原激活剂（u-PA）的纤溶酶原激活剂抑制剂（PA-I）。该抑制剂通过离子交换色谱、凝胶过滤和快速蛋白质液相色谱法进行分离。通过SDS-PAGE测定，该抑制剂的表观分子量为45 kDa，pI为5.5 - 5.7。该抑制剂是一种快速反应抑制剂，热不稳定，在pH范围7 - 9之外会失活。在37℃将胞质溶胶处理至pH 9 30分钟会导致抑制活性大幅增加。针对人胎盘UK-I的抗体完全消除了人白细胞UK-I的抑制活性。
