Maeta Noritaka, Iwai Ryosuke, Takemitsu Hiroshi, Akashi Natsuki, Miyabe Masahiro, Funayama-Iwai Marina, Nakayama Yasuhide
Faculty of Veterinary Medicine, Okayama University of Science, 1-3 Ikoi-no-Oka, Imabari 794-8555, Ehime, Japan.
Institute of Frontier Science and Technology, Okayama University of Science, 1-1 Ridaicho, Kita-ku 700-0005, Okayama, Japan.
Bioengineering (Basel). 2024 Apr 28;11(5):435. doi: 10.3390/bioengineering11050435.
The administration of mesenchymal stem cells (MSCs) has a positive effect on wound healing; however, the lack of adequate MSC engraftment at the wound site is a major limiting factor in current MSC-based therapies. In this study, a biosheet prepared using in-body tissue architecture (iBTA) was used as a material to address these problems. This study aimed to assess and evaluate whether biosheets containing somatic stem cells would affect the wound healing process in dogs. Biosheets were prepared by subcutaneously embedding molds in beagles. These were then evaluated grossly and histologically, and the mRNA expression of inflammatory cytokines, interleukins, and Nanog was examined in some biosheets. Skin defects were created on the skin of the beagles to which the biosheets were applied. The wound healing processes of the biosheet and control (no biosheet application) groups were compared for 8 weeks. Nanog mRNA was expressed in the biosheets, and SSEA4/CD105 positive cells were observed histologically. Although the wound contraction rates differed significantly in the first week, the biosheet group tended to heal faster than the control group. This study revealed that biosheets containing somatic stem cells may have a positive effect on wound healing.
间充质干细胞（MSCs）的施用对伤口愈合具有积极作用；然而，伤口部位缺乏足够的MSCs植入是当前基于MSCs的治疗方法的一个主要限制因素。在本研究中，使用体内组织结构（iBTA）制备的生物片作为解决这些问题的材料。本研究旨在评估含有体细胞干细胞的生物片是否会影响犬类的伤口愈合过程。通过在比格犬皮下植入模具来制备生物片。然后对其进行大体和组织学评估，并检测一些生物片中炎性细胞因子、白细胞介素和Nanog的mRNA表达。在比格犬的皮肤上制造皮肤缺损，并将生物片应用于其上。比较生物片组和对照组（未应用生物片）的伤口愈合过程，为期8周。生物片中表达了Nanog mRNA，组织学观察到SSEA4/CD105阳性细胞。尽管在第一周伤口收缩率有显著差异，但生物片组的愈合速度倾向于比对照组更快。本研究表明，含有体细胞干细胞的生物片可能对伤口愈合有积极作用。
