Yan Jun, He Lizhong, Huang Zhiwu, Wang Hong, Yu Li, Zhu Weimin
Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Key Laboratory of Protected Horticulture Technology, Shanghai 201403, China.
Foods. 2024 May 7;13(10):1428. doi: 10.3390/foods13101428.
Celery seeds contain various bioactive compounds and are commonly used as a spice and nutritional supplement in people's daily lives. The quality of celery seeds sold on the market varies, and their regions of production are unclear. This study evaluated the metabolites of Chinese celery seeds from three production regions using HS-SPME-GC-MS, HS-GC-IMS, and UPLC-ESI-MS/MS. The results indicate that GC-IMS analysis obtained a metabolic profile different from that detected using GC-MS. Terpenoids, polyphenols, coumarins, and phthalides are the main bioactive compounds in celery seeds. The production region significantly affects the metabolic characteristics of celery seeds. Based on GC-MS data, GC-IMS data, and LC-MS data, the variation analysis screened 6, 12, and 8 metabolites as potential characteristic metabolites in celery seeds related to the production region, respectively. According to the aromatic characteristics of the characteristic metabolites, seeds from the HCQ region and HZC region have a strong herbal, woody, celery, and turpentine aroma. The concentration of secondary metabolites was highest in the seeds from the HCQ region followed by the HZC region, and it was the lowest in the JJC region. Altogether, this study investigates how geographical origins influence the metabolomic profile of celery seeds. The results can be used to guide the planting and harvesting of celery seeds in suitable regions.
芹菜籽含有多种生物活性化合物，在人们的日常生活中常用作香料和营养补充剂。市场上销售的芹菜籽质量参差不齐，其产地也不明确。本研究采用顶空固相微萃取-气相色谱-质谱联用（HS-SPME-GC-MS）、顶空气相色谱-离子迁移谱（HS-GC-IMS）和超高效液相色谱-电喷雾串联质谱（UPLC-ESI-MS/MS）对来自三个产地的中国芹菜籽的代谢物进行了评估。结果表明，气相色谱-离子迁移谱分析得到的代谢谱与气相色谱-质谱检测的不同。萜类化合物、多酚、香豆素和苯酞是芹菜籽中的主要生物活性化合物。产地显著影响芹菜籽的代谢特征。基于气相色谱-质谱数据、气相色谱-离子迁移谱数据和液相色谱-质谱数据，变异分析分别筛选出6种、12种和8种代谢物作为与产地相关的芹菜籽潜在特征代谢物。根据特征代谢物的香气特征，来自HCQ地区和HZC地区的种子具有浓郁的草本、木质、芹菜和松节油香气。次生代谢物的浓度在HCQ地区的种子中最高，其次是HZC地区，在JJC地区最低。总之，本研究调查了地理来源如何影响芹菜籽的代谢组学特征。研究结果可用于指导芹菜籽在适宜地区的种植和收获。
