Polcaro Luciana Maria, Samani Marzieh Rahmani, Piacente Sonia, Masullo Milena
Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II, 84084 Salerno, Italy.
PhD Program in Drug Discovery and Development, Università degli Studi di Salerno, Via Giovanni Paolo II n. 132, 84084 Fisciano, Italy.
Foods. 2024 May 7;13(10):1429. doi: 10.3390/foods13101429.
The pomegranate ( L.) market has steadily grown due to the increasing demand of health-conscious consumers of products with health-promoting effects. Recently, a growing interest in developing ecological and environmentally friendly extraction methods has led to investigating extraction procedures to obtain a higher extraction yield using a lower amount of solvents and energy. Herein, a new extraction procedure was developed to obtain an enriched pomegranate food supplement by using the peels of pomegranate, cultivar "Dente di Cavallo" and its juice. The extraction was performed through a non-conventional extraction technique like SLDE-Naviglio using ethanol and pomegranate juice as a solvent, and peels as a matrix. The extract was analysed by a combined approach based on LCESI/QExactive/MS/MS and NMR analysis, and its chemical profile was compared with those of pomegranate juice and of the extract obtained from peels by SLDE-Naviglio by using ethanol:HO. The LC-MS analysis highlighted the presence of hydrolysable tannins, flavonoids, ellagic acid and phenol glucoside derivatives, while H NMR analysis completed the profile by detecting the primary metabolites. The LC-MS and H NMR analysis indicated that the extract obtained by SLDE-Naviglio using ethanol and pomegranate juice was enriched in the bioactives as confirmed by the highest phenolic, tannin and flavonoid content.
由于注重健康的消费者对具有促进健康功效产品的需求不断增加，石榴（L.）市场稳步增长。最近，人们对开发生态环保型提取方法的兴趣日益浓厚，这促使人们研究提取工艺，以便用较少的溶剂和能源获得更高的提取率。在此，开发了一种新的提取工艺，以石榴品种“Dente di Cavallo”的果皮及其果汁为原料，制备富含石榴的食品补充剂。提取过程通过一种非常规提取技术进行，如使用乙醇和石榴汁作为溶剂、果皮作为基质的SLDE-Naviglio技术。提取物采用基于LCESI/QExactive/MS/MS和NMR分析的组合方法进行分析，并将其化学特征与石榴汁以及使用乙醇:水从果皮中通过SLDE-Naviglio获得的提取物的化学特征进行比较。LC-MS分析突出了可水解单宁、黄酮类化合物、鞣花酸和酚类糖苷衍生物的存在，而1H NMR分析通过检测初级代谢产物完善了化学特征。LC-MS和1H NMR分析表明，使用乙醇和石榴汁通过SLDE-Naviglio获得的提取物富含生物活性成分，这一点通过最高的酚类、单宁和黄酮类含量得到证实。
