Moeurng Sreylak, Posridee Kakanang, Kamkaew Anyanee, Thaiudom Siwatt, Oonsivilai Anant, Oonsivilai Ratchadaporn
Department of Bioengineering, Faculty of Engineering, Royal University of Phnom Penh, Phnom Penh 12156, Cambodia.
Health and Wellness Research Group, School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Foods. 2024 May 7;13(10):1443. doi: 10.3390/foods13101443.
Linn. (Rang Chuet, RC), a Thai medicinal plant, possesses various bioactive compounds with potential health benefits. This study aimed to identify detoxifying compounds within RC crude extract. RC leaves were extracted using the Soxhlet method with chloroform. Total carotenoids, chlorophylls, extract yield, total phenolic contents (TPCs), and total flavonoid contents (TFCs) were measured. The extract's composition was analyzed. Cytotoxicity and effects on the detoxification enzyme NQO-1 were assessed in liver cell lines (AML12 and HepG2) using MTT and NQO-1 assays, respectively. Bioactive fractions were identified using fractionation techniques and mass spectrometry (LC-MS). RC extract displayed significant levels of carotenoids (0.375 mg/g), chlorophylls (2.682 mg/g), and favorable yield (15.3%). TPC and TFC were 363.776 mg/g and 112.22 mg/g of extract, respectively. Analysis revealed phenolic acids (gallic acid, caffeic acid), flavonoid (apigenin), chlorophylls (chlorophylls , , pheophytin and ), and lutein. Among the fractions, Fraction 3 (F3) exhibited the highest NQO-1 enzyme activity. F3 contained pheophytin and hydroxy pheophytin , confirmed by LC-MS (/ 871.59 [M + H] and 887.59 [M + H]). F3 significantly induced NQO-1 activity in both HepG2 (3.908-fold) and AML12 (1.99-fold) cells. This study identified F3 from RC extract as a promising fraction containing pheophytin and hydroxy pheophytin , responsible for inducing the detoxification enzyme NQO-1 in liver cells. These findings suggest RC's potential for promoting detoxification.
林奈（Rang Chuet，RC）是一种泰国药用植物，含有多种具有潜在健康益处的生物活性化合物。本研究旨在鉴定RC粗提物中的解毒化合物。采用索氏提取法用氯仿提取RC叶片。测定了总类胡萝卜素、叶绿素、提取物得率、总酚含量（TPC）和总黄酮含量（TFC）。分析了提取物的成分。分别使用MTT和NQO-1测定法在肝细胞系（AML12和HepG2）中评估细胞毒性和对解毒酶NQO-1的影响。使用分馏技术和质谱（LC-MS）鉴定生物活性组分。RC提取物显示出较高水平的类胡萝卜素（0.375毫克/克）、叶绿素（2.682毫克/克）和良好的得率（15.3%）。提取物的TPC和TFC分别为363.776毫克/克和112.22毫克/克。分析发现了酚酸（没食子酸、咖啡酸）、黄酮类（芹菜素）、叶绿素（叶绿素a、叶绿素b、脱镁叶绿素a和脱镁叶绿素b）和叶黄素。在这些组分中，组分3（F3）表现出最高的NQO-1酶活性。F3含有脱镁叶绿素a和羟基脱镁叶绿素a，经LC-MS确认（m/z 871.59 [M + H]和887.59 [M + H]）。F3在HepG2（3.908倍）和AML12（1.99倍）细胞中均显著诱导NQO-1活性。本研究确定RC提取物中的F3是一种有前景的组分，含有脱镁叶绿素a和羟基脱镁叶绿素a，可在肝细胞中诱导解毒酶NQO-1。这些发现表明RC具有促进解毒的潜力。
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