Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Parasitol Res. 2012 Jul;111(1):353-9. doi: 10.1007/s00436-012-2846-5. Epub 2012 Feb 11.
Thunbergia laurifolia Linn (Rang Chuet) possesses antioxidant and anti-inflammatory properties as well as anticancer activities. The aim of the present study was to evaluate the efficacy of T. laurifolia in reducing inflammation from pathological changes in Syrian hamsters infected with the human liver fluke Opisthorchis viverrini. Hamster groups were also administered N-nitrosodimethylamine (NDMA) and treated with T. laurifolia. Light microscopic observation of histopathological changes, liver function tests for alanine transaminase (ALT) and alkaline phosphatase (ALP) and kidney function tests for blood urea nitrogen (BUN) and creatinine were performed. Antioxidant effects of both fresh and dried Rang Chuet solutions were observed. Analysis of the histopathological changes showed anti-inflammatory properties, both in the case of O. viverrini infection or with NDMA administration, by reducing the aggregation of inflammatory cells surrounding the hepatic bile ducts as indicated by normal serum ALT, ALP, BUN and creatinine levels in treated Syrian hamsters. The present study found that fresh and dried Rang Chuet solutions clearly reduced the inflammatory cells in both O. viverrini-infected and NDMA-administered groups and was correlated with the total antioxidant capacity. These findings suggest that T. laurifolia possesses antioxidant and anti-inflammatory properties and that its application may be useful for prevention of the inflammatory process, one of the risk factors of O. viverrini-associated cholangiocarcinoma (CCA).
山乌龟（Rang Chuet）具有抗氧化和抗炎特性以及抗癌活性。本研究旨在评估山乌龟在减轻感染华支睾吸虫（Opisthorchis viverrini）的叙利亚仓鼠病理变化引起的炎症中的功效。还向仓鼠组给予 N-亚硝基二甲胺（NDMA）并进行山乌龟治疗。进行肝组织病理学变化的光镜观察、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）的肝功能测试以及血尿素氮（BUN）和肌酐的肾功能测试。观察了新鲜和干燥的 Rang Chuet 溶液的抗氧化作用。组织病理学变化分析表明，无论是感染华支睾吸虫还是给予 NDMA，山乌龟都具有抗炎特性，通过降低肝内胆管周围炎性细胞的聚集来实现，这表现为治疗后的叙利亚仓鼠的血清 ALT、ALP、BUN 和肌酐水平正常。本研究发现，新鲜和干燥的 Rang Chuet 溶液可明显减少华支睾吸虫感染和 NDMA 给药组中的炎性细胞，与总抗氧化能力相关。这些发现表明山乌龟具有抗氧化和抗炎特性，其应用可能有助于预防炎症过程，这是华支睾吸虫相关胆管癌（CCA）的一个危险因素。
