Hematyar Nima, Rahimnejad Samad, Gorakh Waghmare Swapnil, Malinovskyi Oleksandr, Policar Tomas
Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25 Vodňany, Czech Republic.
Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
Foods. 2024 May 10;13(10):1477. doi: 10.3390/foods13101477.
There is currently insufficient acknowledgment of the relationship between fish welfare and ultimate fillet quality. The purpose of this study was to assess the impacts of pre-slaughter handling and stocking density as fish welfare markers on fillet quality of largemouth bass (). Fish from three stocking densities of 35, 50, and 65 kg·m were reared in a recirculating aquaculture system (RAS) for 12 weeks and received commercial feed. Ultimately, the fish were either stunned with percussion on the head (control group) or subjected to air exposure for 3 min (anoxia group) before stunning and subsequent collection of blood and fillet samples. Western blot analysis revealed the degradation of actin in both groups. Additionally, higher oxidation progress and lower hardness and pH were observed in anoxia compared to the control group. We observed higher hardness at 35 kg·m in anoxia compared to 50 and 65 km. The initial hardness values at 35, 50, and 65 km were 1073, 841, and 813 (g) respectively in the anoxia group. Furthermore, the anoxia and control groups had rigor mortis after 6 and 10 h, respectively. Cortisol and glucose levels, and oxidative enzymes activity were higher in anoxia than in the control group. In conclusion, oxidation induced by anoxia likely plays a crucial role as a promoter of the quality deterioration of largemouth bass fillets.
目前，人们对鱼类福利与最终鱼片品质之间的关系认识不足。本研究的目的是评估作为鱼类福利指标的宰前处理和放养密度对大口黑鲈鱼片品质的影响。将三种放养密度分别为35、50和65 kg·m³的鱼在循环水养殖系统（RAS）中饲养12周，并投喂商业饲料。最终，对鱼进行处理，对照组通过敲击头部使其昏迷，缺氧组在昏迷前进行3分钟的空气暴露（缺氧处理），随后采集血液和鱼片样本。蛋白质免疫印迹分析显示两组中肌动蛋白均有降解。此外，与对照组相比，缺氧组观察到更高的氧化进程、更低的硬度和pH值。我们观察到，在缺氧组中，35 kg·m³放养密度下的鱼片硬度高于50和65 kg·m³放养密度下的鱼片。在缺氧组中，35、50和65 kg·m³放养密度下的初始硬度值分别为1073、841和813（g）。此外，缺氧组和对照组分别在6小时和10小时后出现尸僵。缺氧组的皮质醇和葡萄糖水平以及氧化酶活性均高于对照组。总之，缺氧诱导的氧化可能是大口黑鲈鱼片品质劣化的关键促进因素。
