Lou Yingyue, Jia Rui, Li Bing, Zhou Linjun, Zhu Jian, Hou Yiran
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Animals (Basel). 2024 Dec 15;14(24):3618. doi: 10.3390/ani14243618.
Snail is not only useful for bioremediation, purifying aquaculture environments, but it is also a commercially valuable and nutritionally rich aquatic product. To analyze the effect of various stocking densities on the muscle nutritional quality and metabolic functions of . The transcriptome and metabolome were analyzed and set up three different density groups-low (LD, 234.38 g/m), medium (MD, 468.75 g/m), and high (HD, 937.5 g/m). The results of the study showed that the weight gain (WG) and specific growth rate (SGR) of in the MD and HD groups were significantly lower compared to the LD group. High stocking density significantly reduced the oleic acid (C18:1n9c), linoleic acid (C18:2n6c), alpha-linolenic acid (C18:3n3), eicosadienoic acid (C20:2), erucic acid (C22:1n9), docosahexaenoic acid (DHA, C22:6n3), and lignoceric acid (C24:0) levels within snail foot muscle. Most of the identified differentially expressed genes (DEGs) were categorized as Signal transduction, according to the Kyoto Encyclopedia of Genes and Genomes (KEGG); these genes were categorized into Transport and catabolism, Endocrine system, and Immune system. A total of 11 upregulated DEGs and 19 downregulated DEGs were identified and confirmed to be associated with density stress. The identified metabolites were mainly enriched in the Metabolism category, with 620 differential metabolites identified in positive ion (POS) mode and 265 differential metabolites identified in the negative ion (NEG) mode among different stocking density groups. The differential metabolites affected by stocking density were primarily amino acids, nucleic acids, vitamins, and lipid metabolites. There were 8 upregulated differential metabolites and 14 downregulated differential metabolites identified and confirmed to be associated with density stress. These findings elucidated the response mechanisms of to adverse stocking density conditions and provide data and a theoretical basis for selecting appropriate stocking densities for .
田螺不仅对生物修复、净化水产养殖环境有用，而且还是一种具有商业价值且营养丰富的水产品。为分析不同放养密度对田螺肌肉营养品质和代谢功能的影响。对转录组和代谢组进行了分析，并设置了三个不同密度组——低密度（LD，234.38克/平方米）、中密度（MD，468.75克/平方米）和高密度（HD，937.5克/平方米）。研究结果表明，MD组和HD组田螺的体重增加（WG）和特定生长率（SGR）显著低于LD组。高放养密度显著降低了田螺足部肌肉中的油酸（C18:1n9c）、亚油酸（C18:2n6c）、α-亚麻酸（C18:3n3）、二十碳二烯酸（C20:2）、芥酸（C22:1n9）、二十二碳六烯酸（DHA，C22:6n3）和木蜡酸（C24:0）水平。根据京都基因与基因组百科全书（KEGG），大多数鉴定出的差异表达基因（DEG）被归类为信号转导；这些基因被归类为运输和分解代谢、内分泌系统和免疫系统。共鉴定出11个上调的DEG和19个下调的DEG，并证实它们与密度应激有关。鉴定出的代谢物主要富集在代谢类别中，不同放养密度组中在正离子（POS）模式下鉴定出620种差异代谢物，在负离子（NEG）模式下鉴定出265种差异代谢物。受放养密度影响的差异代谢物主要是氨基酸、核酸、维生素和脂质代谢物。共鉴定出8个上调的差异代谢物和14个下调的差异代谢物，并证实它们与密度应激有关。这些发现阐明了田螺对不利放养密度条件的响应机制，并为选择合适的田螺放养密度提供了数据和理论依据。
