Centro de Tecnología Biomédica (CTB), Universidad Politécnica de Madrid (UPM), 28223 Madrid, Spain.
Escuela Técnica Superior de Ingenieros de Telecomunicación (ETSIT), Universidad Politécnica de Madrid (UPM), 28040 Madrid, Spain.
Int J Mol Sci. 2024 May 7;25(10):5074. doi: 10.3390/ijms25105074.
Since the establishment of regulations for exposure to extremely low-frequency (0-300) Hz electromagnetic fields, scientific opinion has prioritised the hypothesis that the most important parameter determining cellular behaviour has been intensity, ignoring the other exposure parameters (frequency, time, mode, waveform). This has been reflected in the methodologies of the in vitro articles published and the reviews in which they are included. A scope review was carried out, grouping a total of 79 articles that met the proposed inclusion criteria and studying the effects of the different experiments on viability, proliferation, apoptosis, oxidative stress and the cell cycle. These results have been divided and classified by frequency, intensity, exposure time and exposure mode (continuous/intermittent). The results obtained for each of the processes according to the exposure parameter used are shown graphically to highlight the importance of a good methodology in experimental development and the search for mechanisms of action that explain the experimental results, considering not only the criterion of intensity. The consequence of this is a more than necessary revision of current exposure protection regulations for the general population based on the reductionist criterion of intensity.
自制定 0-300 赫兹极低频电磁场暴露法规以来,科学界优先假设决定细胞行为的最重要参数是强度,而忽略了其他暴露参数(频率、时间、模式、波形)。这反映在已发表的体外文章的方法和其中包含的综述中。进行了范围审查,共分组了 79 篇符合建议纳入标准的文章,并研究了不同实验对活力、增殖、细胞凋亡、氧化应激和细胞周期的影响。这些结果按频率、强度、暴露时间和暴露模式(连续/间歇)进行了划分和分类。根据使用的暴露参数,为每个过程获得的结果以图形方式显示,以强调在实验开发中采用良好方法的重要性,以及寻找解释实验结果的作用机制,而不仅仅考虑强度标准。其结果是,基于强度还原论标准,对普通人群的现行暴露保护法规进行了比必要的更多修订。
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