Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
Int J Mol Sci. 2024 May 18;25(10):5526. doi: 10.3390/ijms25105526.
This work aimed to study the effect of repeated exposure to low doses of ozone on alpha-synuclein and the inflammatory response in the , jejunum, and colon. Seventy-two male Wistar rats were divided into six groups. Each group received one of the following treatments: The control group was exposed to air. The ozone groups were exposed for 7, 15, 30, 60, and 90 days for 0.25 ppm for four hours daily. Afterward, they were anesthetized, and their tissues were extracted and processed using Western blotting, immunohistochemistry, and qPCR. The results indicated a significant increase in alpha-synuclein in the and jejunum from 7 to 60 days of exposure and an increase in NFκB from 7 to 90 days in the , while in the jejunum, a significant increase was observed at 7 and 15 days and a decrease at 60 and 90 days for the colon. Interleukin IL-17 showed an increase at 90 days in the in the jejunum and increases at 30 days and in the colon at 15 and 90 days. Exposure to ozone increases the presence of alpha-synuclein and induces the loss of regulation of the inflammatory response, which contributes significantly to degenerative processes.
本研究旨在探讨重复低剂量臭氧暴露对 、空肠和结肠中α-突触核蛋白和炎症反应的影响。将 72 只雄性 Wistar 大鼠分为 6 组。每组接受以下一种处理：对照组暴露于空气中。臭氧组每天暴露于 0.25 ppm 臭氧 4 小时，分别暴露 7、15、30、60 和 90 天。之后，对大鼠进行麻醉，提取其组织，并用 Western blot、免疫组织化学和 qPCR 进行处理。结果表明，暴露 7 至 60 天时，α-突触核蛋白在 和空肠中的表达显著增加，NFκB 从 7 天增加到 90 天；而在空肠中，7 天和 15 天明显增加，60 天和 90 天减少；在结肠中，IL-17 在 90 天的 中增加，在 30 天的空肠和 15 天和 90 天的结肠中增加。臭氧暴露增加了α-突触核蛋白的存在，并诱导炎症反应失去调节，这对退行性过程有重要贡献。
Zotero 插件
