Xia Yingjun, Wang Zhaoyang, Hu Yanli, Zhao Pengfei, Li Jianhai, Zhang Li, Fang Rui, Zhao Junlong
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan 430070, China.
Microorganisms. 2024 Apr 30;12(5):917. doi: 10.3390/microorganisms12050917.
() is a zoonotic pathogen capable of causing severe diseases in humans and pigs, including meningitis, sepsis, polyserositis, arthritis, and endocarditis. This study aimed to investigate the biological characteristics of 19 strains of isolated from diseased pigs in Hubei Province between 2021 and 2023. Through bioinformatics analysis, we investigated the serotype, MLST, pan-genome characteristics, SNP, AMR, and ICE of the 19 isolates. Among the 19 strains, ten serotypes were identified, and serotype 9 was the most prevalent (21.05%). Ten new alleles and nine new sequence types (STs) were discovered, with ST28 and ST243 emerging as the predominant STs. The results of the pan-genomic analysis of indicate that there are 943 core genes, 2259 shell genes, and 5663 cloud genes. Through SNP evolutionary analysis, we identified a strong genetic similarity between SS31 and the reference genome P1/7. The analysis of antibiotic resistance genes revealed widespread presence of (B) and (O) genes among 19 strains of . This association may be linked to the high resistance of to lincosamides, macrolides, and tetracyclines. Integrative and conjugative elements (ICEs) and integrative and mobilizable elements (IMEs) were identified in 16 strains, with a carriage rate of 84.21%, and resistance genes were identified within the ICE/IME elements of 8 strains. Antimicrobial susceptibility testing revealed that all strains showed sensitivity to vancomycin and lincomycin but resistance to tilmicosin, tiamulin, amoxicillin, and doxycycline. This study contributes to our understanding of the genomic diversity of in Hubei Province of China, providing essential data for the comprehensive prevention and control of infections in China.
（）是一种人畜共患病原体，可导致人类和猪的严重疾病，包括脑膜炎、败血症、多浆膜炎、关节炎和心内膜炎。本研究旨在调查2021年至2023年期间从湖北省患病猪中分离出的19株（）的生物学特性。通过生物信息学分析，我们研究了这19株分离株的血清型、多位点序列分型（MLST）、泛基因组特征、单核苷酸多态性（SNP）、抗菌药物耐药性（AMR）和整合性接合元件（ICE）。在这19株菌株中，鉴定出了10种血清型，血清型9最为常见（21.05%）。发现了10个新等位基因和9种新序列型（STs），其中ST28和ST243成为主要的STs。（）的泛基因组分析结果表明，有943个核心基因、2259个外壳基因和5663个云基因。通过SNP进化分析，我们确定SS31与参考基因组P1/7之间存在很强的遗传相似性。抗生素抗性基因分析显示，在19株（）中广泛存在（B）和（O）基因。这种关联可能与（）对林可酰胺类、大环内酯类和四环素类药物的高耐药性有关。在16株菌株中鉴定出了整合性接合元件（ICEs）和整合性可移动元件（IMEs），携带率为84.21%，并且在8株菌株的ICE/IME元件中鉴定出了抗性基因。药敏试验表明，所有菌株对万古霉素和林可霉素敏感，但对替米考星、泰妙菌素、阿莫西林和强力霉素耐药。本研究有助于我们了解中国湖北省（）的基因组多样性，为中国（）感染的综合防控提供重要数据。
